Optical beam spectrometer with movable lens
A spectroscopic system is described that provides at least one of focus of an excitation beam onto a sample, automatic focus of an optical system of the spectroscopic system for collecting a spectroscopic signal, and/or averaging of excitation intensity over a surface area of the sample.
1. A Raman spectroscopic system, comprising:
a laser light source adapted to provide a beam of illumination;
an optical system comprising an optical element adapted to focus the beam of illumination on a sample and receive light from the sample;
a scanner adapted to scan the beam over an area of the sample;
and
a controller adapted to average Raman spectroscopic measurements taken from the light from the sample while the scanner scans the beam over an area of the sample and control the focus of the optical system by summing up groups from the Raman spectroscopic measurements and comparing the resulting sums.
2. The Raman spectroscopic system according to claim 1 wherein the spectroscopic system comprises a hand-held spectrometer.
3. The Raman spectroscopic system according to claim 1 , further comprising a Rayleigh filter.
4. The Raman spectroscopic system according to claim 1 , wherein the light from the sample includes background fluorescence and wherein the controller is further adapted to remove the background fluorescence.
5. The spectroscopic system according to claim 4 wherein the controller removes the background fluorescence by calculating a derivative spectrum.
6. The Raman spectroscopic system according to claim 1 , wherein the optical element comprises a prism, and wherein the scanner is adapted to move the prism in an orthogonal direction to the spectroscopic axis of the Raman spectrograph.
7. The Raman spectroscopic system according to claim 6 , wherein the prism comprises a wedge prism.
8. The Raman spectroscopic system according to claim 1 , wherein the optical element comprises a lens.
9. The Raman spectroscopic system according to claim 8 , wherein the scanner is adapted to move the lens in an orthogonal direction to the spectroscopic axis of the Raman spectrometer.
10. The Raman spectroscopic system according to claim 1 , wherein the controller is further adapted to plot a Raman parameter of value.
11. The Raman spectroscopic system according to claim 10 , wherein the Raman parameter of value is selected from a spectral correlation, a shift in Raman band, and intensity of a Raman feature.
12. The Raman spectroscopic system according to claim 1 , wherein the scanner is adapted to scan the beam at a rate faster than the acquisition time of the Raman spectrometer.
13. A method for performing Raman spectroscopy using a Raman spectrograph, comprising:
providing a beam of illumination from a light source;
focusing the beam of illumination on a sample
scanning the beam over an area of the sample;
receiving a signal from the sample;
taking a plurality of spectroscopic measurements while scanning the beam, and averaging the plurality of measurements; and,
controlling the focus of the optical system by summing up groups from the plurality of spectroscopic measurements and comparing the resulting sums.
14. The method according to claim 13 , wherein the step of scanning comprises scanning the beam at a rate faster than the acquisition time of the Raman spectrograph.
15. The method of claim 13 , further comprising plotting a Raman parameter of value.
16. The method of claim 13 , further comprising plotting at least one of: a spectral correlation, a shift in Raman band, and intensity of a Raman feature.
17. The method of claim 13 , further comprising filtering background fluorescence from the signal.
18. The method of claim 13 , further comprising calculating a derivative spectrum.
19. The method of claim 13 , wherein the step of scanning the beam comprises moving a prism in an orthogonal direction to the spectroscopic axis of the Raman spectrometer.
20. The method of claim 13 , wherein the step of scanning the beam comprises moving a lens in an orthogonal direction to the spectroscopic axis of the Raman spectrograph.