Lyase enzymes, nucleic acids encoding them and methods for making and using them
View Patent ↗This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. X═NO2, Cl, Br, NH2, OH, H, alkyl at one or several o, m, and p positions R═H or alkyl.
1. A method for deaminating a phenylalanine, tyrosine, or a histidine comprising the following steps:
(a) providing a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more, or 100% sequence identity to SEQ ID NO 32, or a fragment thereof, or a polypeptide encoded by a nucleic acid having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more, or 100% sequence identity to SEQ ID NO 31;
(b) providing a composition comprising a phenylalanine, tyrosine, or a histidine residue; and
(c) contacting the polypeptide or the fragment thereof of step (a) with the composition of step (b) under conditions wherein the polypeptide or the fragment thereof deaminates the phenylalanine, tyrosine, or a histidine residue in the composition;
wherein the polypeptide or the fragment thereof has lyase activity.