Centrifugal device and method for performing binding assays
The invention relates to a device for performing binding assays. In particular, the invention relates to a centrifugal device for performing such assays. The invention also relates to a method of performing binding assays involving antigen-antibody binding, nucleic acid hybridization, or receptor-ligand interaction.
1. A device for measuring the binding of a first partner in an interaction to a second partner in said interaction, wherein said interaction partners are molecular entities, said device comprising:
a) an opaque temperature-controlled chamber having a centrifuge rotor therein, said rotor having at or near the periphery of the rotor and attached thereto at least one radially positioned transparent reaction well, said reaction well having on an upper surface thereof an aperture for the addition of reagents to the reaction well, said reaction well further including on an internal surface thereof at the end closest the axis of said rotor at least one attachment zone for said second interaction partner,
b) a stationary system for detecting light emitted or absorbed by said first interaction partner or an indicator molecule bound thereto; and
c) means for controlling the temperature of said chamber and the operation of said rotor.
2. The device of claim 1 , wherein said chamber has a lid or scalable opening to allow loading of reaction wells.
3. The device of claim 1 , wherein said temperative control is effected by providing a heater linked to a temperature sensor so that a set temperature can be maintained.
4. The device of claim 1 , wherein said temperature control includes a cooling system.
5. The device of claim 1 , wherein said rotor comprises a fiat disc of a plastic or metal material having said at least one reaction well fitted therein.
6. The device of claim 1 , wherein said rotor has from 1 to 96 reaction wells.
7. The device of claim 1 , wherein said at least one reaction well is manufactured from polypropylene or polycarbonate.
8. The device of claim 1 , wherein said at least one reaction well is cylindrical or a rectangular prism.
9. The device of claim 1 , wherein said at least one reaction well is angled upwards toward the periphery of the rotor.
10. The device of claim 1 , wherein said attachment zone is provided by spotting the second interaction partner onto said internal surface of the reaction well.
11. The device of claim 1 , wherein said attachment zone is provided by way of said second interaction partner being-linked to a magnetic particle which is held in the attachment zone by a magnet.
12. The device of claim 1 , wherein said attachment zone is circular with a diameter of SO um to 3 mm.
13. The device of claim 1 , wherein said drive means is a direct-coupled AC motor, a DC motor or stepper motor with the motor external to the chamber.
14. The device of claim 1 , wherein said light source is an LED, a laser light source or a halogen lamp.
15. The device of claim 1 , wherein said device further includes a computer for controlling an operation selected from the group consisting of rotor speed, chamber temperature, time and temperature for annealing and polymerization steps when the binding assay is a hybridization, rotor braking, rotor vibration, and data processing.