Recombinant yeast host cell with Fe-S cluster proteins and methods of using thereof
View Patent ↗Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
1. A method of converting 2,3-dihydroxyisovalerate to α-ketoisovalerate, comprising providing a recombinant yeast host cell expressing a heterologous dihydroxy-acid dehydratase (DHAD) protein that comprises
(i) an amino acid sequence having at least 95% identity to SEQ ID NO: 179 or 187; and
(ii) three conserved cysteine residues that correspond to positions 56, 129, and 201 of SEQ ID NO:179;
wherein the substrate 2,3-dihydroxyisovalerate is present in the yeast host cell and wherein the expressed heterologous DHAD protein catalyzes the conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate.
2. The method of claim 1 , wherein the amino acid sequence of the heterologous DHAD protein comprises SEQ ID NO: 179.
3. The method of claim 1 , wherein the amino acid sequence of the heterologous DHAD protein comprises SEQ ID NO: 187.
4. The method of claim 1 , wherein the recombinant yeast host cell comprises a disruption in an ilv3 gene that encodes mitochondrial DHAD, resulting in reduced expression of endogenous mitochondrial DHAD.
5. The method of claim 1 , wherein the recombinant yeast host cell comprises an engineered isobutanol biosynthetic pathway.
6. The method of claim 5 , wherein the engineered isobutanol biosynthetic pathway comprises the following substrate to product conversions:
(i) pyruvate to acetolactate (pathway step a);
(ii) acetolactate of (i) to 2,3-dihydroxyisovalerate (pathway step b);
(iii) 2,3-dihydroxyisovalerate of (ii) to α-ketoisovalerate (pathway step c);
(iv) α-ketoisovalerate of (iii) to isobutyraldehyde (pathway step d); and
(v) isobutyraldehyde of (iv) to isobutanol (pathway step e),
and wherein
(a) the substrate to product conversion of step (i) is catalyzed by an acetolactate synthase;
(b) the substrate to product conversion of step (ii) is catalyzed by a ketol-acid reductoisomerase;
(c) the substrate to product conversion of step (iii) is catalyzed by the heterologous DHAD;
(d) the substrate to product conversion of step (iv) is catalyzed by an α-keto acid decarboxylase; and
(e) the substrate to product conversion of step (v) is catalyzed by an alcohol dehydrogenase.
7. The method of claim 6 , wherein two or more of the enzymes (a), (b), and (d) are heterologous to the recombinant yeast host cell.
8. The method of claim 7 , wherein two or more of the enzymes (a), (b), and (d) are over-expressed in the recombinant yeast host cell.
9. The method of claim 1 , wherein the yeast host cell is selected from the group consisting of Saccharomyces, Schizosaccharomyces, Hansenula, Candida, Kluyveromyces, Yarrowia , and Pichia.
10. The method of claim 9 , wherein the yeast host cell is Saccharomyces cerevisiae.
11. The method of claim 6 , further comprising:
(a) culturing the recombinant yeast host cell under suitable conditions to produce isobutanol from pyruvate; and
(b) recovering the isobutanol.
12. The method of claim 11 , wherein said recovering is by distillation, liquid-liquid extraction, adsorption, decantation, pervaporation or combinations thereof.
13. The method of claim 11 , further comprising (c) removing solids from the fermentation medium.
14. The method of claim 13 , wherein said removing is by centrifugation, filtration or decantation.
15. The method of claim 13 , wherein said removing step (c) occurs before said recovering step (b).