Microscope apparatus
View Patent ↗Illuminating light is two-dimensionally scanned without changing the ability to focus the illuminating light on a specimen. Provided is a microscope apparatus including a spatial light modulator that modulates the wavefront of illuminating light; a scanner that two-dimensionally scans the illuminating light by pivoting two mirrors; a relay optical system that relays an image in the scanner to a pupil position of an objective optical system; and a beam-shift mechanism that moves rays of the illuminating light between the modulator and the objective optical system in response to pivoting of the mirrors. The beam-shift mechanism moves the rays such that the image at the pupil position, when assuming that the mirrors are stationary, is moved in the direction opposite to the direction in which the image relayed to the pupil position by the relay optical system, when assuming that the mirrors are pivoted with the rays fixed, is moved.
1. A microscope apparatus comprising:
a spatial light modulator that modulates a wavefront of illuminating light from a light source;
a scanner that has two mirrors independently pivoted about two non-parallel axes and that two-dimensionally scans the illuminating light whose wavefront has been modulated by the spatial light modulator;
a relay optical system that relays an image in the scanner to a pupil position of an objective optical system; and
a beam-shift mechanism that moves rays of the illuminating light in a direction intersecting an optical axis, in response to pivoting of the mirrors, between the spatial light modulator and the objective optical system,
wherein the beam-shift mechanism moves the rays such that the image at the pupil position of the objective optical system, when assuming that the mirrors are stationary, is moved in a direction opposite to a direction in which the image relayed to the pupil position of the objective optical system by the relay optical system, when assuming that the mirrors are pivoted with the rays fixed, is moved, and
wherein the beam-shifting mechanism moves at least one lens of the relay optical system to keep the illuminating light relayed to the pupil position of the objective optical system stationary, regardless of the pivoting of the two mirrors.
2. The microscope apparatus according to claim 1 , wherein the beam-shift mechanism moves at least one of optical devices constituting the relay optical system in a direction intersecting the optical axis.
3. The microscope apparatus according to claim 1 , wherein the beam-shift mechanism causes at least one of optical devices constituting the relay optical system to pivot about a pivot axis intersecting the optical axis.
4. The microscope apparatus according to claim 1 , wherein the relay optical system relays the image between the two mirrors to the pupil position.
5. The microscope apparatus according to claim 1 , wherein the relay optical system relays the image on the pivot axis of one of the mirrors to the pupil position.
6. The microscope apparatus according to claim 5 , wherein the relay optical system relays the image on the pivot axis of the mirror that is pivoted at a higher speed among the two mirrors to the pupil position.
7. The microscope apparatus according to claim 1 , further comprising another relay optical system that relays an image on the spatial light modulator to the scanner.
8. The microscope apparatus according to claim 1 , further comprising another relay optical system that relays an image on the spatial light modulator to the scanner,
wherein the beam-shift mechanism moves at least one of optical devices constituting the other relay optical system in a direction intersecting the optical axis.
9. The microscope apparatus according to claim 1 , further comprising another relay optical system that relays an image on the spatial light modulator to the scanner,
wherein the beam-shift mechanism causes at least one of optical devices constituting the other relay optical system to pivot about a pivot axis intersecting the optical axis.