Method of arraying ferritin
View Patent ↗A method of selectively arraying ferritin and inorganic particles on a silicon oxide substrate at regions having vanadium, niobium or tantalum. An aspect of the method includes steps of: preparing a solution which contains ferritin modified at an N-terminal part of a subunit with a peptide set out in SEQ ID NO: 1, and from 0.01 v/v % to 10 v/v % of a nonionic surfactant and having a pH of from 7.4 to 8.2; and a binding step of bringing the solution in contact with regions of the substrate having vanadium, niobium, or tantalum to selectively array peptide-modified ferritin to vanadium, niobium or, tantalum portion. The method may also include a step of selectively arraying ferritin modified with the peptide set out in SEQ ID NO: 1, and the inorganic particles contained in ferritin at the vanadium, niobium, or tantalum portion by removing the solution.
1. A method of arraying ferritin, the method comprising steps of:
a preparing step of preparing a solution which contains ferritin modified at an N-terminal of a subunit with a peptide set out in SEQ ID NO: 1, wherein a serine residue at the N-terminal of the subunit is bonded to an alanine residue of the peptide of SEQ ID NO: 1, and from 0.01 v/v % to 10 v/v % of a nonionic surfactant, wherein the solution has a pH falling within the range of from 7.4 to 8.2; and
a binding step of bringing the solution in contact with a silicon oxide substrate having a metal portion of one metal selected from vanadium, niobium, and tantalum formed on a part of the surface so as to selectively bind the ferritin to the metal portion.
2. The method according to claim 1 , further comprising after the binding step, a removal step of removing the solution to leave the ferritin selectively bound to the metal portion on the silicon oxide substrate.
3. The method according to claim 1 , further comprising after the binding step, a cleaning step of washing the surface of the silicon oxide substrate with a solution not containing the ferritin so as to leave the ferritin selectively bound to the metal portion at the metal portion on the silicon oxide substrate.
4. The method according to claim 1 , wherein the ferritin contains inorganic particles.
5. The method according to claim 4 , further comprising after the binding step, a decomposing step of heating the silicon oxide substrate to decompose the ferritin to array the inorganic particles which had been contained in the ferritin at the metal portion on the silicon oxide substrate.