Lenalidomide and thalidomide immunoassays
Novel conjugates and immunogens derived from lenalidomide and antibodies generated by these immunogens are useful in immunoassays for the quantification and monitoring of thalidomide and lenalidomide in biological fluids.
1. An antibody which has substantially selective reactivity with both chemotherapeutic drugs lenalidomide and thalidomide or a combination thereof.
2. The antibody of claim 1 wherein said antibody of has a cross reactivity with the non-pharmaceutically active metabolites of said pharmaceutically active chemotherapeutic drugs of not greater than 10%, said cross-reactivity being relative to said antibody's binding to said pharmaceutically active chemotherapeutic drugs.
3. The antibody of claim 2 wherein said antibody is a monoclonal antibody.
4. The antibody of claim 2 wherein said antibody is generated from an immunogen comprising an immunogenic carrier containing a polyamine polymer conjugated with a ligand of the formula:
wherein B is —C(═O)—CH 2 —, —C(═O)—NH—CH 2 —, —C(═O)—O—CH 2 — or —CH 2 —;
Y is an organic spacing group;
p is an integer from 0 to 1; and
X is a terminal functional group capable of binding to said polyamine.
5. The antibody of claim 4 , wherein said antibody is a monoclonal antibody.
6. The antibody of claim 5 , wherein said antibody is derived from mice, sheep, rabbits or rats.
7. The antibody of claim 2 wherein said antibody has selectively reactive with thalidomide of at least 10% based upon its reactivity with said chemotherapeutic drugs.
8. The antibody claim 7 wherein said antibody is a monoclonal antibody.
9. The antibody of claim 8 wherein said antibody is generated from an immunogen comprising an immunogenic carrier containing a polyamine polymer conjugated with a ligand of the formula:
Wherein B is —C(═O)—CH 2 —, —C(═O)—NH—CH 2 —, —C(═O)—O—CH 2 — or —CH 2 —
Y is an organic spacing group;
p is an integer from 0 to 1;
X is a terminal functional group capable of binding to said polyamine polymer.