Resistance to auxinic herbicides
View Patent ↗The invention provides methods of identifying herbicidal auxins. The invention further provides auxin-herbicide-resistant plants and genes conferring auxin-herbicide resistance. This invention also provides a method of identifying other proteins that bind picolinate auxins from additional plant species. The invention further provides a method to identify the molecular binding site for picolinate auxins. The invention also includes the use of the picolinate herbicidal auxin target site proteins, and methods of discovering new compounds with herbicidal or plant growth regulatory activity. The invention also includes methods for producing plants that are resistant to picolinate herbicidal auxins. Specific examples of novel proteins associated with herbicide binding include AFB5, AFB4, and SGT1b.
1. A method of identifying an herbicidal compound that binds with an AFB5 protein, the AFB5 protein comprising a polypeptide comprising an amino acid sequence having at least 90% amino acid sequence identity with SEQ ID NO: 2, the method comprising:
contacting the AFB5 protein with a test compound;
assaying the AFB5 protein for bound compound; and
screening the compound for herbicidal activity.
2. The method of claim 1 , wherein the AFB5 protein comprises a polypeptide comprising an amino acid sequence having 95% amino acid sequence identity with SEQ ID NO: 2.
3. The method of claim 1 , wherein the AFB5 protein comprises a polypeptide comprising an amino acid sequence set forth as SEQ ID NO: 2.
4. The method of claim 1 , wherein the AFB5 protein consists of a polypeptide having an amino acid sequence set forth as SEQ ID NO: 2.
5. The method of claim 1 , wherein the herbicidal compound is an auxin herbicidal compound.
6. The method of claim 1 , wherein the herbicidal compound is a picolinate auxin herbicidal compound.