Risperidone immunoassay
Novel conjugates and immunogens derived from risperidone and antibodies generated by these immunogens are useful in immunoassays for the quantification and monitoring of risperidone and paliperidone in biological fluids.
1. An antibody which is selectively reactive with a pharmaceutically active anti-psychotic drugs risperidone and paliperidone, wherein said antibody has little substantial reactivity with the pharmacologically inactive risperidone and paliperidone metabolite, 7-hydroxy-risperidone, and does not have substantial cross-reactivity with other pharmacologically inactive risperidone and paliperidone metabolites.
2. The antibody of claim 1 wherein the reactivity of said antibody with said 7-hydroxy-risperidone is less than 40% and said cross-reactivity with said other pharmaceutically inactive metabolites is less than 5%, said reactivity and cross reactivity being based upon the antibody's combined reactivity with both risperidone and paliperidone.
3. The antibody of claim 2 wherein said antibody is generated from an immunogen comprising an immunogenic carrier containing a polyamine polymer conjugated with a ligand of the formula:
wherein B is
Y is an organic spacing group;
X is a terminal functional group capable of binding to an immunogenic carrier containing a polyamine polymer; and
p is an integer from 0 to 1.
4. The antibody of claim 3 , wherein said antibody is derived from mice, sheep, rabbits or rats.
5. The antibody of claim 3 , wherein said antibody is a monoclonal antibody.