PHA-producing bacteria
The present disclosure relates to isolated bacterial strains producing polyhydroxyalkanoate (PHA), microbial consortia including such strains, and cultures of such strains and microbial consortia. In particular, the present disclosure relates to compositions including such strains, microbial consortia, and cultures and methods of use thereof.
1. One or more isolated polyhydroxyalkanoate (PHA)-producing bacterial cells of bacterial strain Delftia acidovorans MM01 deposited with ATCC as Accession No. PTA-12280.
2. The one or more bacterial cells of claim 1 , wherein said cells produce at least 10 grams of PHA per 100 grams dry weight of said cells when said cells are grown in a biogenic waste feed stream at an oxygen concentration of about 0.0038 milligrams per liter of biogenic waste feed stream at 20° C. to about 1.14 milligrams per liter of biogenic waste feed stream at 20° C. a temperature range of about 15° C. to about 34° C., and a pH that ranges from about 6.5 to about 11, wherein said biogenic waste feed stream comprises one or more organic acids.
3. The one or more bacterial cells of claim 1 , wherein the one or more organic acids are selected from the group consisting of acetic acid, propanoic acid, 2-methylpropanoic acid, 2,2-dimethylpropanoic acid, butanoic acid, 2-methylbutanoic acid, 3-methylbutanoic acid, pentanoic acid, hexanoic acid, caproic acid, caprylic acid, capric acid, and laurie acid.
4. The one or more bacterial cells of claim 1 , wherein said one or more bacterial cells comprise at least 20 grams of PHA per 100 grams dry weight of said cells.
5. The one or more bacterial cells of claim 1 , wherein said PHA is poly 3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV), medium-chain-length PHA (mcl-PHA), short-chain-length PHA (scl-PHA), or combinations thereof.
6. The one or more bacterial cells of claim 5 , wherein said scl-PHA is polyhydroxybutyrate (PHB).
7. The one or more bacterial cells of claim 1 , wherein said PHA is poly 3-hydroxybutyrate-co-3-hydroxyvalerate (PBV).
8. A composition comprising the one or more bacterial cells of claim 1 .
9. The composition of claim 8 further comprising one or more strains, wherein said one or more bacterial cells promote the ability of said one or more strains to produce PHA.
10. An isolated microbial consortium comprising the one or more bacterial cells of claim 1 .
11. The isolated microbial consortium of claim 10 , further comprising one or more strains, wherein said one or more bacterial cells promote the ability of said one or more strains to produce PHA.
12. A composition comprising the isolated microbial consortium of claim 10 .