Synthetic, defined fibronectin mimetic peptides and surfaces modified with the same
View Patent ↗The present invention discloses compositions for applications that mimic fibronectin coated surfaces. Advantageously, such compositions provide an animal free (xeno-free, and human-component-free), synthetic, chemically defined surface that mimics at least one of the functionalities of fibronectin.
1. A compound comprising at least two regions of a fibronectin cell-binding domain joined by a flexible linker wherein at least one region comprises amino acid sequence GRGDSP (SEQ ID NO: 1) and another region comprises X 1 X 2 X 3 X 4 X 5 X 6 X 7 X 8 X 9 X 10 X 11 X 12 X 13 RX 15 PX 17 SRNX 21 X 22 TLTX 26 (SEQ ID NO: 2) wherein
X 1 denotes K or is absent,
X 2 denotes K or is absent,
X 3 denotes K or is absent,
X 4 denotes K or is absent,
X 5 denotes K or is absent,
X 6 denotes K,
X 7 denotes S, V, A, G or is absent
X 8 denotes G, A or is absent
X 9 denotes R or G
X 10 denotes P, A, Q or G
X 11 denotes R or K
X 12 denotes E, Q, W, A or G
X 13 denotes D or E
X 15 denotes V, L or I
X 17 denotes H or P
X 21 denotes S, T or G
X 22 denotes I or L and
X 26 denotes N or H; and wherein the compound is less than 100 amino acids.
2. The compound of claim 1 wherein X 7 denotes S.
3. The compound of claim 2 wherein X 8 denotes G.
4. The compound of claim 3 wherein X 9 denotes R.
5. The compound of claim 1 wherein the flexible linker comprises amino acid sequence SGSGSGSGS (SEQ ID NO: 3), GGSGGSGGS (SEQ ID NO: 4), SGTGSGTGS (SEQ ID NO: 5) or GGGSGGGSGG (SEQ ID NO: 6).
6. The compound of claim 1 wherein
X 1 is absent,
X 2 is absent,
X 3 is absent,
X 4 is absent,
X 5 is absent,
X 7 denotes S
X 8 denotes G
X 9 denotes R
X 10 denotes P
X 11 denotes R
X 12 denotes E
X 13 denotes D
X 15 denotes V
X 17 denotes H
X 21 denotes S
X 22 denotes I and
X 26 denotes N.
7. The compound of claim 6 wherein the flexible linker is SGSGSGSGS (SEQ ID NO: 3).
8. A surface wherein at least a portion of the surface comprises a coating thereon of claim 1 .