Methods For Cell Culture Using A Synthetic, Defined Collagen Mimetic Surface
The present invention discloses methods for enhancing cell attachment, cell proliferation and cell function using a surface which mimics a collagen coated surface. Advantageously, such methods employ a xeno-free, synthetic, chemically defined surface.
1 . A method for cell culture comprising contacting a suspension of cells to a surface wherein at least a portion of the surface comprises a coating thereon of a compound comprising amino acid sequence GPCGPPGPPGPPGPPGPPGFX 1 GERGPPGPPGPPGPPGPPGPC (SEQ ID NO: 1) wherein X 1 represents hydroxyproline and incubating the cells under conditions suitable for cell culture; wherein cell attachment to the surface is comparable to cell attachment of the suspension of cells to a collagen coated surface.
2 . The method of claim 1 , wherein the cells are keratinocytes.
3 . The method of claim 1 , wherein the cells are pancreatic cancer cells.
4 . The method of claim 1 , wherein the cells are hepatocytes.
5 . The method of claim 1 , wherein the number of cells attached to the surface is at least 2-fold greater relative to a control surface without any extracellular matrix protein coating thereon.
6 . The method of claim 2 , wherein the number of cells attached to the surface is at least 3-fold greater relative to a control surface without any extracellular matrix protein coating thereon.
7 . The method of claim 3 , wherein the number of cells attached to the surface is at least 10-fold greater relative to a control surface without any extracellular matrix protein coating thereon.
8 . The method of claim 4 , wherein the level of basal activity of cytochrome P 450 3A4 in hepatocytes attached to the surface is comparable to the level of basal activity of cytochrome P 450 3A4 in hepatocytes attached to a collagen coated surface.
9 . The method of claim 4 , wherein cytochrome P 450 3A4 induction is comparable to the level of cytochrome P450 induction of hepatocytes attached to a collagen coated surface.
10 . The method of claim 1 , wherein the surface is a cell culture vessel.
11 . The method of claim 1 , wherein the surface is a microcarrier.
12 . The method of claim 1 , wherein the cells are incubated at 37° C. in a humidified incubator with 5% CO 2 .
13 . The method of claim 1 , wherein the cells are incubated for at least 24 hours.
14 . A cell cultured using the method of claim 1 .