Closed bioreactor
Single-use closed bioreactors with recirculating exhaust gas that can be operated in an uncontrolled environment are reported for the manufacturing of biological products using genetically modified biological cultures that produces carbon dioxide or that requires carbon dioxide in their metabolic process.
1. A closed bioreactor, suitable for use in uncontrolled environment, comprising:
a) a container comprising a nutrient medium and a biological culture;
b) at least one gas outlet tube comprising an in line cartridge connected to the gas outlet comprising a carbon dioxide absorbent material;
c) at least one gas inlet connected to the container comprising a tube for introducing gas into the container and wherein the gas inlet is also connected to the gas outlet through a tube allowing the gas to recirculate within the container, and wherein the gas inlet is connected to a source of gas;
d) at least one liquid port for adding liquid to the container, wherein the liquid port is connected to the container and comprises a tube extending below the surface of the nutrient medium;
e) a sterilizing filter capable of removing contaminants installed between the source of gas and the gas inlet;
f) a stopcock installed between the sterilizing filter (e) and the gas inlet;
g) a sterilizing filter capable of removing contaminants installed at the liquid port;
h) a stopcock installed between sterilizing filter (g) and the liquid port;
i) a peristaltic pump installed in the tube connected between the gas outlet and the gas inlet; and
j) at least one sparger connected to the gas inlet and located in the container.
2. The closed bioreactor according to claim 1 , further comprising an outer support housing providing support to said container.
3. The closed bioreactor according to claim 1 , further comprising a filter drain capable of retaining particles larger than 5μ.
4. The closed bioreactor according to claim 1 , wherein the biological culture is a human cell, an animal cell, a bacterium, a plant cell, yeast, or fungi.
5. The closed bioreactor according to claim 1 , further comprising a plurality of remotely operated sensors.
6. The closed bioreactor according to claim 5 , wherein the remotely operated sensors comprise oxygen and carbon dioxide sensors.
7. The closed bioreactor according to claim 1 , further comprising a temperature controller for the nutrient medium.
8. The closed bioreactor according to claim 1 , wherein the container is comprised of a material that is disposable.
9. The closed bioreactor according to claim 1 , wherein the container is comprised of a flexible polymer.
10. The closed bioreactor according to claim 1 , wherein the container comprises a biocompatible polymer.
11. The closed bioreactor according to claim 1 , wherein the carbon dioxide absorbent material is selected from the group of absorbent materials comprising soda lime, calcium hydroxide, sodium hydroxide, potassium hydroxide, lithium hydroxide, lithium silicate, lithium zirconate, lithium oxide, activated charcoal, zeolite and an ion exchange resin.