Methods and compositions for diagnosis of thyroid conditions
The present invention relates to compositions, kits, and methods for molecular profiling and cancer diagnostics, including but not limited to genomic DNA markers associated with cancer. In particular, the present invention provides molecular profiles associated with thyroid cancer, methods of determining molecular profiles, and methods of analyzing results to provide a diagnosis.
1. A method for diagnosing thyroid disease in a subject, the method comprising:
(a) providing a DNA sample from a subject;
(b) detecting in the DNA sample the presence of one or more polymorphisms of one or more genes selected from IYD, PSD4, Clorf68, KPRP, and LCE1E, wherein the one or more polymorphisms comprise one or more variations in copy number as compared to a normal sample; and
(c) determining whether said subject has or is likely to have a malignant or benign thyroid condition based on the results of step (b).
2. The method of claim 1 , wherein the malignant thyroid condition is selected from the group consisting of follicular carcinoma, follicular variant of papillary carcinoma, and papillary thyroid carcinoma.
3. The method of claim 1 , wherein the benign thyroid condition is selected from the group consisting of follicular adenoma, and nodular hyperplasia.
4. The method of claim 1 , wherein the DNA sample provided from said subject is obtained from a sample comprising thyroid tissue.
5. The method of claim 1 , wherein the variation in copy number as compared to a normal sample comprises a deletion.
6. The method of claim 1 , wherein the variation in copy number as compared to a normal sample comprises an increase in the copy number.
7. The method of claim 1 , wherein the normal sample comprises a sample of DNA from the same subject.
8. The method of claim 1 , wherein the normal sample comprises a sample of DNA from a different subject.
9. The method of claim 1 , wherein the detecting step (b) comprises:
(a) contacting said DNA sample with one or more binding agents that specifically bind to the one or more polymorphisms; and
(b) determining whether said DNA sample specifically binds to said one or more binding agents, wherein binding of said DNA sample to said one or more binding agents indicates the presence of the one or more polymorphisms in said subject.
10. The method of claim 1 , wherein the detecting step (b) comprises sequencing of one or more nucleic acid regions comprising said one or more polymorphisms.
11. The method of claim 1 , wherein the detecting step (b) comprises quantifying the amount of DNA comprising said one or more polymorphisms.
12. The method of claim 11 , wherein the quantifying comprises PCR.
13. The method of claim 12 , wherein the PCR comprises real-time PCR.
14. The method of claim 11 , wherein the quantifying comprises hybridization.
15. The method of claim 1 , wherein the method further comprises determining the expression level of one or more genes correlated with follicular adenoma, follicular carcinoma, nodular hyperplasia, follicular variant of papillary carcinoma, or papillary thyroid carcinoma.
16. The method of claim 1 , wherein the method further comprises providing a therapeutic intervention to the subject based on a result of (c).
17. The method of claim 1 comprising detecting two or more polymorphisms selected from PAX8, IYD, PSD4, Clorf68, KPRP, and LCE1E.
18. The method of claim 1 comprising detecting copy number increase of one or more genes selected from IYD, PSD4, C1orf68, KPRP, and LCE1E.
19. The method of claim 18 further comprising determining whether said subject has or is likely to have a malignant or benign thyroid condition, wherein said determining is based on the copy number increase of one or more of said genes in said DNA sample.
20. The method of claim 1 wherein the method further comprises performing cytological and/or histological testing on a sample from the subject.
21. The method of claim 20 wherein the sample for cytological and/or histological testing is the same as the sample for providing a DNA sample.
22. The method of claim 20 wherein the cytological testing and/or histological testing is inconclusive as to whether the subject has a benign or a malignant thyroid condition.