Hide processing methods and compositions
View Patent ↗Provided in part herein are compositions that include isolated microbial enzymes, such as proteases, lipases, useful in dehairing leather. Also provided are methods for isolating proteases, lipases, from microbes, as well as methods of reusing these enzymes effectively for several cycles in an environment friendly process.
1. A rawhide dehairing process, comprising:
contacting a rawhide with a solution including a lipase and a protease for a predetermined amount of time, wherein the pH of the solution is from 5-10; and wherein the lipase is a Pseudomonas aeruginosa strain B/GZN secreted lipase; and removing hair from the rawhide.
2. A rawhide dehairing process, comprising:
contacting a rawhide with a solution including a Pseudomonas aeruginosa lipase and a protease for a predetermined amount of time, wherein the pH of the solution is from 5-10; and wherein the protease is a Pseudomonas aeruginosa strain A/SRC002 secreted protease; and removing hair from the rawhide.
3. The process of claim 1 , wherein the protease is a bacterial protease.
4. The process of claim 1 , wherein the protease is from a gram positive bacteria.
5. The process of claim 1 , wherein the protease is from a gram negative bacteria.
6. The process of claim 3 , wherein the protease is a Pseudomonas aeruginosa secreted protease.
7. The process of claim 6 , wherein the protease is a Pseudomonas aeruginosa strain A/SRC002 secreted protease.
8. The process of claim 1 , further comprising mechanically removing hair from the rawhide.
9. The process of claim 8 , further comprising scrubbing the rawhide.
10. The process of claim 1 , wherein the rawhide is contacted with the solution for 10 hours or more.
11. The process of claim 1 , wherein the pH is about 7.5 to about 8.0.
12. The process of claim 1 , wherein the pH is about pH 8.0.
13. The process of claim 1 , wherein the solution comprises Pseudomonas aeruginosa strain B/GZN.
14. The process of claim 13 , wherein the Pseudomonas aeruginosa strain B/GZN is immobilized or suspended in culture.
15. The process of claim 14 , wherein the Pseudomonas aeruginosa strain B/GZN is immobilized in a porous matrix.
16. The process of claim 14 , wherein the Pseudomonas aeruginosa strain B/GZN is immobilized in sodium alginate with calcium chloride.
17. The process of claim 1 , wherein the rawhide is salted, dried, or both salted and dried.
18. The process of claim 1 , wherein the solution is maintained at a temperature of about 40° C. for the predetermined amount of time.
19. The process of claim 1 , wherein the solution is applied to the rawhide by one or more of painting, dipping or spraying.
20. The process of claim 1 , wherein the solution is reusable.
21. The process of claim 20 , wherein the solution is reusable for at least 7 cycles or at least 20 cycles.
22. The process of claim 2 , wherein the lipase is a bacterial lipase.
23. The process of claim 2 , wherein the lipase is from a gram positive bacteria.
24. The process of claim 2 , wherein the lipase is from a gram negative bacteria.
25. The process of claim 22 , wherein the lipase is a Pseudomonas aeruginosa secreted lipase.
26. The process of claim 25 , wherein the lipase is a Pseudomonas aeruginosa strain B/GZN secreted lipase.
27. The process of claim 2 , further comprising mechanically removing hair from the rawhide.
28. The process of claim 27 , further comprising scrubbing the rawhide.
29. The process of claim 2 , wherein the rawhide is contacted with the solution for 10 hours or more.
30. The process of claim 2 , wherein the pH is about 7.5 to about 8.0.
31. The process of claim 2 , wherein the pH is about pH 8.0.
32. The process of claim 2 , wherein the solution comprises Pseudomonas aeruginosa strain A/SRC002.
33. The process of claim 32 , wherein the Pseudomonas aeruginosa strain A/SRC002 is immobilized or suspended in culture.
34. The process of claim 33 , wherein the Pseudomonas aeruginosa strain A/SRC002 is immobilized in a porous matrix.
35. The process of claim 33 , wherein the Pseudomonas aeruginosa strain A/SRC002 is immobilized in sodium alginate with calcium chloride.
36. The process of claim 2 , wherein the rawhide is salted, dried, or both salted and dried.
37. The process of claim 2 , wherein the solution is maintained at a temperature of about 40° C. for the predetermined amount of time.
38. The process of claim 2 , wherein the solution is applied to the rawhide by one or more of painting, dipping or spraying.
39. The process of claim 2 , wherein the solution is reusable.
40. The process of claim 39 , wherein the solution is reusable for at least 7 cycles or at least 20 cycles.