Use of midkine protein and the protein-containing medical device
The applications and medical devices of Midkine in the field of biotechnology are described in this invention. This invention describes the applications of the protein as follow in (a) or (b) in preparing drugs for promoting cartilage growth or treating cartilage diseases, the protein as a growth factor for promoting chondrocyte proliferation, or medical devices containing this protein: (a) the protein whose amino acid sequence is shown as SEQ ID NO.1; (b) the proteins whose amino acid sequence have at least 60% homology comparing with amino acid sequence described in (a). The present invention is different from the present knowledge of MK and its related proteins. The experimental results demonstrated that MK promotes proliferation of three types of chondrocytes and is used for preparing drugs for treating cartilage disease. It may provide new therapy for treating cartilage diseases clinically, and new technology for expansion of chondrocytes in vitro for cartilage tissue engineering.
1. A method for treating a degenerative cartilage disease in a subject in need thereof, the method comprising administering to the subject at a site in need of treatment an effective amount of a protein consisting of the amino acid sequence of SEQ ID NO: 1, wherein the degenerative cartilage disease is selected from the group consisting of osteoarthritis and intervertebral disc degeneration, wherein the effective amount is 10 μg/kg/day to 300 μg/kg/day.
2. The method of claim 1 , wherein the administering comprises implanting into the subject a medical device which comprises an effective amount of the protein.
3. The method of claim 2 , wherein the medical device is in the form of a scaffold which comprises an effective amount of the protein.
4. The method of claim 3 , wherein the scaffold comprises collagen IIa.
5. The method of claim 1 , wherein the effective amount is 300 μg/kg/day.
6. A method for treating traumatic cartilage damage in a subject in need thereof, the method comprising administering to the subject at a site in need of treatment an effective amount of a protein consisting of the amino acid sequence of SEQ ID NO: 1, wherein the effective amount is 10 μg/kg/day to 300 μ/kg/day.
7. The method of claim 6 , wherein the effective amount is 300 μg/kg/day.