Split spin centrifugation of test elements
View Patent ↗High-throughput centrifugation of batches of samples is achieved by dividing a single centrifugation run of a batch of samples into two or more staggered, discrete spins with fewer samples and in which the availability of centrifuge slots for loading or unloading of samples and the subsequent delivery of experimental results can be enhanced. The methodology is particularly useful in situations where multiple samples need to be processed rapidly, for example, as part of a STAT blood typing program in urgent care facilities prior to blood transfusion.
1. A testing apparatus, comprising:
a) a plurality of centrifuges configured for the centrifugation of plurality of sample receptacles;
b) one or more drive mechanisms, said drive mechanisms connected to said sample centrifuges;
c) at least one transfer mechanism configured for the loading or unloading of said sample centrifuges, and
d) a control mechanism interfaced with said drive mechanisms and said at least one transfer mechanism, said control mechanism configured for the staggered operation of said centrifuges, wherein said staggered operation comprises the steps of:
i. creating a plurality of smaller secondary batches from a primary batch of test samples, wherein said primary batch typically requires centrifugation for a time period equal to t in a primary centrifuge and the number of secondary batches is equal to y, which is at least 2;
ii. loading the test samples of said secondary batches into x number of separate secondary centrifuges, in which x is at least 2;
iii. staggering the operation of said x number of secondary centrifuges by a time period t/xy in relation to one another over a total centrifugation cycle for each secondary centrifuge that is equal to time period t;
iv. pausing the operation of each of said staggered secondary centrifuges;
v. at least one of loading new test samples and unloading completed test samples from each of said paused secondary centrifuges during said pausing step; and
vi. resuming said centrifugation of said paused secondary centrifuges, wherein the frequency of said unloading and reloading of each of said secondary centrifuges with said secondary batches is increased xy fold as compared to the frequency of unloading and reloading of a primary centrifuge loaded with a primary batch of test samples.
2. The testing apparatus of claim 1 , wherein said test samples are disposed within sample receptacles.
3. The testing apparatus of claim 1 , further comprising one or more detectors configured to detect particle agglutination reactions within said sample receptacles.
4. The testing apparatus of claim 1 , wherein said sample receptacles are labeled with a bar code.
5. The testing apparatus of claim 1 , further comprising a bar code reader.
6. The testing apparatus of claim 1 , further comprising one or more incubators configured to modulate the temperature of said samples.
7. The testing apparatus of claim 1 , wherein said unloading and loading occurs every t/xz+z seconds, wherein z equals the number of seconds required for said loading and unloading.
8. The testing apparatus of claim 7 , wherein z equals from 1 to 120 seconds.
9. The testing apparatus of claim 1 , wherein said sample receptacles are bead cassettes.
10. The testing apparatus of claim 9 , wherein said bead cassettes are gel cards.
11. The testing apparatus of claim 1 , wherein said sample receptacles contain human samples.
12. The testing apparatus of claim 1 , wherein said sample receptacles contain human blood samples.
13. The testing apparatus of claim 1 , wherein said apparatus is configured for rapid processing of one or more emergency samples.
14. The testing apparatus of claim 1 , wherein said sample receptacles contain reagents for particle agglutination assays.
15. The testing apparatus of claim 1 , wherein said sample receptacles comprise reagents for blood typing.
16. The testing apparatus of claim 1 , wherein the number of secondary centrifuges is from 2 to 10.
17. The testing apparatus of claim 1 , wherein the number of secondary batches is from 2 to 10.
18. The testing apparatus of claim 1 , wherein the number of sample receptacles in said secondary batch is from 2 to 100.
19. The testing apparatus of claim 1 , wherein the frequency of reloading of said secondary centrifuges with said secondary batches is increased from 2 to 40 fold as compared to the frequency of reloading of the primary centrifuge with said primary batch of sample receptacles.
20. The testing apparatus of claim 1 , wherein said testing apparatus is configured to assess said sample receptacles in each of said secondary batches for a result after centrifuging each of said secondary batches for t/x number of seconds.