Method of treating an osteochondral defect
View Patent ↗The present invention is directed to compositions having at least one neocartilage particle, juvenile cartilage particle or a combination thereof and a matrix, and methods and devices that include the compositions.
1. A method of treating an ostechondral defect, comprising:
disposing an osteo-conductive matrix in the defect;
implanting cadaveric, allogenic human juvenile cartilage particles including viable chondrocytes into the defect; and
depositing a chondro-conductive matrix into the defect.
2. The method of claim 1 , wherein the osteo-conductive matrix is disposed in the defect prior to implanting the cartilage particles.
3. The method of claim 1 or 2 , wherein the cartilage particles are implanted and the chondro-conductive matrix is deposited simultaneously.
4. The method of claim 3 , wherein the cartilage particles and the chondro-conductive matrix comprise a single construct.
5. The method of claim 4 , wherein a preformed single construct is produced by delivering the cartilage particles and the chondro-conductive matrix to a mold.
6. The method of claim 4 , wherein the preformed single construct is attached to the osteo-conductive matrix or cartilage or bone adjacent the defect by gluing.
7. The method of claim 3 , wherein the cartilage particles are implanted and the chondro-conductive matrix is deposited through a cannulated device.
8. The method of claim 7 , wherein the cartilage particles and the chondro-conductive matrix are mixed during deposition.
9. The method of claim 1 , wherein the cartilage particles are implanted and the chondro-conductive matrix is deposited sequentially.
10. The method of claim 9 , wherein chondro-conductive matrix is deposited after cartilage particles are implanted into the defect.
11. The method of claim 1 , further comprising applying a retainer to the defect.
12. The method of claim 11 , wherein the retainer comprises a glue.
13. The method of claim 1 , wherein the osteo-conductive matrix is disposed up to the tide mark adjacent the osteochondral defect.
14. The method of claim 1 , wherein the osteo-conductive matrix is selected from the group consisting of a porous solid, semisolid, paste or gel.
15. The method of claim 14 , wherein the osteo-conductive matrix comprises a porous solid.
16. The method of claim 15 , wherein the porous solid comprises an interconnected structure.
17. The method of claim 16 , wherein the porous solid comprises cancellous bone.
18. The method of claim 1 , wherein the osteo-conductive matrix is selected from the group consisting of bone or a calcium phosphate containing composition.
19. The method of claim 18 , wherein the bone is demineralized.
20. The method of claim 1 , further comprising removing the cartilage particles from a container including a biocompatible storage solution.
21. The method of claim 1 , wherein the cartilage particles have a dimension from about one to about three millimeters.
22. The method of any one of claims 1 , 2 , 9 , 11 , 15 or 20 , wherein the cartilage particles range in size from about 1 to about 27 mm 3 .
23. The method of claim 1 , wherein the cartilage particles comprise articular cartilage.
24. The method of claim 1 , wherein the chondro-conductive matrix is selected from the group consisting of plasma-based adhesives or sealants, and protein-based adhesives or sealants, natural adhesives or sealants and any combination thereof.
25. The method of claim 24 , wherein the chondro-conductive matrix is fibrin.
26. The method of claim 1 , 2 , 9 , 11 , 15 or 20 , wherein the cartilage particles are from cadaveric juvenile donors less than fifteen years of age.
27. The method of claim 26 , wherein the cartilage particles are from cadaveric juvenile donors less than two years of age.
28. The method of claim 26 , wherein the cartilage particles are from cadaveric juvenile donors from about 20 weeks to about 13 years of age.