IP Library › Granted Patent US 8,771,708
Granted Patent B2
US 8,771,708 · App. 13/327,842 · Granted Jul 8, 2014

Biologically-active radiolabeled Cry1Fa and receptor binding assay methods

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Quick Facts
Patent No.
US 8,771,708
App. No.
13/327,842
Granted
Jul 8, 2014
Kind
B2
Abstract

Cysteine-specific radiolabeled Cry1Fa protein retains insecticidal activity against insect pests and binds to insect brush border membrane vesicle receptors in a saturable manner. The biologically-active radiolabeled Cry1Fa protein is useful in competitive binding assays with other Cry toxins.

Claims (2)

1. A Cry1Fa holotoxin labeled with radioiodinated fluorescein-5-maleimide at position C205, said radiolabeled holotoxin retaining the ability to selectively bind to receptors in brush border membrane vesicles prepared from midguts of Spodoptera exigua and Spodoptera frugiperda.

2. The Cry1Fa holotoxin of claim 1 , said radiolabeled Cry1Fa holotoxin having insecticidal activity.

Assignments (1)
CHANGE OF NAME Recorded Nov 8, 2021
From: DOW AGROSCIENCES LLC
To: CORTEVA AGRISCIENCE LLC
Reel/Frame 058044/0184 →