Method of preparing neutrophil-depleted platelet-rich plasma
View Patent ↗Methods of producing neutrophil-depleted platelet-rich plasma by passing a blood, platelet or platelet-rich plasma fraction through a narrow, twisted and/or charged environment to remove neutrophils and produce neutrophil-depleted platelet-rich plasma is described. The neutrophil-depleted platelet-rich plasma may be depleted in neutrophils by 75% or more and includes at least 0.5×10 6 platelets per ml. The pH of the neutrophil-depleted platelet-rich plasma may be adjusted to 7.3-7.5.
1. A method of preparing neutrophil-depleted platelet rich plasma comprising:
obtaining a blood sample from a patient,
preparing platelet-rich plasma comprising at least 0.5×10 6 platelets per ml of plasma from the blood sample,
passing the platelet-rich plasma through a cell separation device to remove neutrophils, and
collecting the neutrophil-depleted platelet-rich plasma comprising at least 0.5×10 6 platelets per ml;
wherein the cell separation device comprises:
an inlet end portion comprising an inlet port for receiving a sample of platelet rich plasma;
an outlet end portion comprising at least one collection port for removing neutrophil-depleted platelet rich plasma; and
a flow path placed within an electric field comprising a tubular material in fluid communication with the inlet port and the outlet port;
wherein the tubular material is latticed or coiled and has a diameter of 10-100 microns; and wherein the outlet end has a positive electrical charge and the inlet end has a negative electrical charge.
2. The method according to claim 1 , wherein the tubing is packed into a unit.
3. The method according to claim 2 , wherein the unit is part of a system for concentrating platelets or processing blood.
4. The method according to claim 3 , wherein the system includes a centrifuge.
5. The method according to claim 1 , further comprising adjusting the pH of the neutrophil-depleted platelet-rich plasma to a pH of about 7.3 to 7.5, wherein the neutrophil-depleted platelet-rich plasma composition does not contain an activator of the neutrophil-depleted platelet-rich plasma.
6. The method according to claim 1 , wherein the blood is from an autologous source.
7. The method according to claim 1 , wherein the neutrophil content of the neutrophil-depleted platelet-rich plasma has been reduced by 50-75% compared to the blood or platelet-rich plasma sample.
8. The method according to claim 1 , wherein the neutrophil content of the neutrophil-depleted platelet-rich plasma has been reduced by more than 75% compared to the blood or platelet-rich plasma fraction.
9. A method of preparing neutrophil-depleted platelet rich plasma comprising:
obtaining a blood sample from a patient, passing the blood sample through a cell separation device to remove neutrophils,
preparing platelet-rich plasma comprising at least 0.5×10 6 platelets per ml of plasma from the neutrophil-depleted blood sample, and
collecting the neutrophil-depleted platelet-rich plasma comprising at least 0.5×10 6 platelets per ml;
wherein the cell separation device comprises:
an inlet end portion comprising an inlet port for receiving a sample of platelet rich plasma;
an outlet end portion comprising at least one collection port for removing neutrophil-depleted platelet rich plasma; and
a flow path placed within an electric field comprising a tubular material in fluid communication with the inlet port and the outlet port;
wherein the tubular material is latticed or coiled and has a diameter of 10-100 microns; and wherein the outlet end has a positive electrical charge and the inlet end has a negative electrical charge.
10. The method according to claim 9 , wherein the tubing is packed into a unit.
11. The method according to claim 10 , wherein the unit is part of a system for concentrating platelets or processing blood.
12. The method according to claim 11 , wherein the system includes a centrifuge.
13. The method according to claim 9 , further comprising adjusting the pH of the neutrophil-depleted platelet-rich plasma to a pH of about 7.3 to 7.5, wherein the neutrophil-depleted platelet-rich plasma composition does not contain an activator of the neutrophil-depleted platelet-rich plasma.
14. The method according to claim 9 , wherein the blood is from an autologous source.
15. The method according to claim 9 , wherein the neutrophil content of the neutrophil-depleted platelet-rich plasma has been reduced by 50-75% compared to the blood or platelet-rich plasma sample.
16. The method according to claim 9 , wherein the neutrophil content of the neutrophil-depleted platelet-rich plasma has been reduced by more than 75% compared to the blood or platelet-rich plasma fraction.