Combined use of Vip3Ab and Cry1Ab for management of resistant insects
View Patent ↗The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising a Vip3Ab insecticidal protein in combination with a Cry1Ab protein to delay or prevent development of resistance by the insect(s)—particularly corn earworm.
1. A transgenic plant comprising DNA encoding a Vip3Ab insecticidal protein and DNA encoding a Cry1Ab insecticidal protein, wherein said Cry1Ab insecticidal protein comprises SEQ ID NO:2 and said Vip3Ab insecticidal protein comprises SEQ ID NO:1.
2. Seed of the plant of claim 1 , wherein said seed comprises said DNA encoding said Vip3Ab insecticidal protein and DNA encoding said Cry1Ab insecticidal protein.
3. The plant of claim 1 wherein said DNA was introgressed into said plant.
4. Seed of the plant of claim 3 , wherein said seed contains DNA encoding said Vip3Ab insecticidal protein and DNA encoding said Cry1Ab insecticidal protein.
5. A plurality of transgenic plants of claim 1 in a field of plants comprising non-Bt refuge plants and said transgenic plants, wherein said refuge plants comprise less than 40% of all crop plants in said field.
6. The plants of claim 5 , wherein said refuge plants comprise less than 30% of all the crop plants in said field.
7. The plants of claim 5 , wherein said refuge plants comprise less than 20% of all the crop plants in said field.
8. The plants of claim 5 , wherein said refuge plants comprise less than 10% of all the crop plants in said field.
9. The plants of claim 5 , wherein said refuge plants comprise less than 5% of all the crop plants in said field.
10. The plants of claim 5 , wherein said refuge plants are in blocks or strips.
11. A mixture of seeds comprising refuge seeds from non-Bt refuge plants, and a plurality of seeds of claim 2 , wherein said refuge seeds comprise less than 40% of all the seeds in the mixture.
12. The mixture of seeds of claim 11 , wherein said refuge seeds comprise less than 30% of all the seeds in the mixture.
13. The mixture of seeds of claim 11 , wherein said refuge seeds comprise less than 20% of all the seeds in the mixture.
14. The mixture of seeds of claim 11 , wherein said refuge seeds comprise less than 10% of all the seeds in the mixture.
15. The mixture of seeds of claim 11 , wherein said refuge seeds comprise less than 5% of all the seeds in the mixture.
16. A method of managing development of resistance by Helicoverpa zea insects to an insecticidal protein derived from a Bacillus thuringiensis , said method comprising planting seeds to produce the plants of claim 5 .
17. A composition for controlling Helicoverpa zea insects that are resistant to a Cry protein, said composition comprising cells that express effective amounts of both a Cry1Ab insecticidal protein and a Vip3Ab insecticidal protein.
18. The composition of claim 17 comprising a host transformed to express both a Cry1Ab insecticidal protein and a Vip3Ab insecticidal protein, wherein said host is a microorganism or a plant cell.
19. A method of controlling Helicoverpa zea insects that are resistant to a Cry protein said method comprising presenting to said insects an effective amount of the composition of claim 17 .
20. The plants of claim 5 , wherein said plants occupy more than 10 acres.
21. The plant of claim 1 , wherein said plant is selected from the group consisting of corn, soybeans, and cotton.
22. The plant of claim 1 , wherein said plant is a maize plant.
23. A plant cell of the plant of claim 1 , wherein said plant cell comprises said DNA encoding said Cry1Ab insecticidal protein and said DNA encoding said Vip3Ab insecticidal protein, wherein said Cry1Ab insecticidal protein is SEQ ID NO:2 and said Vip3Ab insecticidal protein is SEQ ID NO:1.
24. A plant cell comprising DNA encoding a Vip3Ab insecticidal protein having SEQ ID NO: 1 and DNA encoding a Cry1Ab insecticidal protein having SEQ ID NO:2.