Modulation of exportin 5 expression
View Patent ↗Compounds, compositions and methods are provided for modulating the expression of exportin 5. The compositions comprise oligonucleotides, targeted to nucleic acid encoding exportin 5. Methods of using these compounds for modulation of exportin 5 expression and for diagnosis and treatment of diseases and conditions associated with expression of exportin 5 are provided.
1. A single-stranded antisense oligomeric compound 13 to 50 nucleobases in length that is at least 90% complementary to a region within nucleotides 4006 to 4527 of SEQ ID NO:4, wherein the compound comprises at least one modified nucleobase, sugar, or internucleoside linkage, and wherein the compound comprises about 6 to about 16 consecutive deoxynucleosides.
2. The compound of claim 1 wherein the compound is at least 90% complementary to a region within nucleotides 4006 to 4025 of SEQ ID NO:4.
3. The compound of claim 1 wherein the compound is at least 90% complementary to a region within nucleotides 4294 to 4313 of SEQ ID NO:4.
4. The compound of claim 1 wherein the compound is at least 90% complementary to a region within nucleotides 4304 to 4323 of SEQ ID NO:4.
5. The compound of claim 1 which is 15 to 30 nucleobases in length.
6. The compound of claim 1 which is a chimeric oligonucleotide.
7. The compound of claim 1 wherein the modified sugar is a 2′-O-methoxyethyl sugar moiety, wherein the modified oligonucleoside linkage is a phosphorothioate internucleoside linkage, and wherein the modified nucleobase is a 5-methylcytosine.
8. An oligomeric compound-RNA duplex wherein the oligomeric compound of the duplex is the compound of claim 1 , and wherein the RNA of the duplex is an RNA that encodes at least the portion of SEQ ID NO: 4 to which the oligomeric compound hybridizes.
9. A composition comprising the compound of claim 1 and a pharmaceutically acceptable salt or carrier.
10. A kit or assay device comprising the compound of claim 1 .
11. A method of inhibiting the expression of exportin 5 in a cell, tissue, or animal comprising contacting the cell, tissue, or animal with the compound of claim 1 .
12. A method of treating an animal having a disease or condition associated with exportin 5 comprising administering to the animal a therapeutically or prophylactically effective amount of the compound of claim 1 so that expression of exportin 5 is inhibited.