Viral promoter, truncations thereof, and methods of use
The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
1. A DNA construct comprising a promoter consisting of the nucleotide sequence of SEQ ID NO: 1 and a heterologous nucleotide sequence of interest operably linked to the promoter.
2. A DNA construct comprising a promoter consisting of the nucleotide sequence of SEQ ID NO: 2 and a heterologous nucleotide sequence of interest operably linked to the promoter.
3. An isolated nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO: 3.
4. A DNA construct comprising the nucleotide sequence of claim 3 operably linked to a heterologous nucleotide sequence of interest.
5. A vector comprising the DNA construct of claim 4 , 1 or 2 .
6. A plant cell having stably incorporated into its genome the DNA construct of claim 4 , 1 or 2 .
7. The plant cell of claim 6 , wherein said plant cell is from a monocot.
8. The plant cell of claim 7 , wherein said monocot is maize.
9. The plant cell of claim 6 , wherein said plant cell is from a dicot.
10. A plant having stably incorporated into its genome the DNA construct of claim 4 , 1 or 2 .
11. The plant of claim 10 , wherein said plant is a monocot.
12. The plant of claim 11 , wherein said monocot is maize.
13. The plant of claim 10 , wherein said plant is a dicot.
14. A transgenic seed of the plant of claim 10 , wherein the seed comprises the DNA construct.
15. The plant of claim 10 , wherein the heterologous nucleotide sequence of interest encodes a gene product that confers herbicide, salt, cold, drought, pathogen, or insect resistance.
16. A method for expressing a nucleotide sequence in a plant, said method comprising introducing into a plant a DNA construct, said DNA construct comprising a promoter nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence consisting of the sequence set forth in SEQ ID NO: 2;
b) a nucleotide sequence consisting of the sequence set forth in SEQ ID NO: 1; and
c) a nucleotide sequence comprising the sequence set forth in SEQ ID NO: 3; and
a heterologous nucleotide sequence of interest operably linked to said promoter.
17. The method of claim 16 , wherein said plant is maize, and wherein said heterologous nucleotide sequence of interest is selectively expressed in green plant tissues.
18. The method of claim 16 , wherein said plant is a dicot.
19. The method of claim 16 , wherein said plant is a monocot.
20. The method of claim 19 , wherein said monocot is maize.
21. The method of claim 16 , wherein the heterologous nucleotide sequence encodes a gene product that confers herbicide, salt, cold, drought, pathogen, or insect resistance.
22. A method for expressing a nucleotide sequence in a plant cell, said method comprising introducing into a plant cell a DNA construct comprising a promoter comprises a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence consisting of the sequence set forth in SEQ ID NO: 2;
b) a nucleotide sequence consisting of the sequence set forth in SEQ ID NO: 1; and
c) a nucleotide sequence comprising the sequence set forth in SEQ ID NO: 3; and
a heterologous nucleotide sequence of interest operably linked to said promoter.
23. The method of claim 22 , wherein said plant cell is from a monocot.
24. The method of claim 23 , wherein said monocot is maize.
25. The method of claim 22 , wherein said plant cell is from a dicot.
26. The method of claim 22 , wherein the heterologous nucleotide sequence encodes a gene product that confers herbicide, salt, cold, drought, pathogen, or insect resistance.
27. A method for selectively expressing a nucleotide sequence in maize green plant tissues, said method comprising introducing into a plant cell a DNA construct comprising a promoter nucleotide sequence selected from the group consisting of:
a) the nucleotide sequence consisting of the sequence set forth in SEQ ID NO: 2,
b) the nucleotide sequence consisting of the sequence set forth in SEQ ID NO: 1, and
c) a nucleotide sequence comprising the sequence set forth in SEQ ID NO: 3; and
a heterologous nucleotide sequence of interest operably linked to said promoter; and
regenerating a transformed plant from said plant cell.
28. The method of claim 27 , wherein expression of said heterologous nucleotide sequence alters the phenotype of said maize plant.
29. The method of claim 27 , wherein the heterologous nucleotide sequence encodes a gene product that confers herbicide, salt, cold, drought, pathogen, or insect resistance.