Counter assembly, sheath flow impedance count device, and flow cytometer analyzer
View Patent ↗A counter assembly, sheath flow impedance count device, and flow cytometer analyzer are disclosed.
1. A counter assembly comprising a counter, a waste liquid isolation chamber, a rear sheath isolation chamber, and a pressure balance tube; wherein
the counter has a front cell, a rear cell, a rear sheath liquid inlet, and a waste liquid outlet;
the front cell and rear cell are in communication through a counting hole;
the rear sheath liquid inlet and the waste liquid outlet are in communication with the rear cell;
the rear sheath isolation chamber and the rear sheath liquid inlet are connected with each other;
the waste liquid isolation chamber is connected with the waste liquid outlet;
the pressure balance tube is connected with the rear sheath isolation chamber and the waste liquid isolation chamber; and
the pressure balance tube has a pressure balance controller.
2. The counter assembly according to claim 1 , wherein
the rear sheath isolation chamber comprises a first cell body and a first liquid adding inlet, the first liquid adding inlet extending downwardly into the first cell body;
the waste liquid isolation chamber comprises a second cell body and a second liquid adding inlet, the second liquid adding inlet extending downwardly into the second cell body; and
a bottom of the first liquid adding inlet is higher than a bottom of the second liquid adding inlet, and a bottom of the first cell body is higher than a bottom of the second cell body.
3. The counter assembly according to claim 2 , wherein the rear sheath isolation chamber further comprises a first top cover disposed at the top of the first cell body, the first liquid adding inlet being disposed at the first top cover, and the first top cover having a first connector;
wherein the waste liquid isolation chamber further comprises a second top cover disposed at the top of the second cell body, the second liquid adding inlet being disposed at the second top cover, the second top cover having a second connector; and
wherein the bottom of the first cell body is connected with the rear sheath liquid inlet, and the pressure balance tube is connected with the first connector and the second connector.
4. The counter assembly according to claim 1 , wherein the rear sheath isolation chamber is in communication with the atmosphere via a gas tube.
5. The counter assembly according to claim 1 , wherein the rear sheath isolation chamber has an oblique guiding surface.
6. The counter assembly according to claim 5 , wherein the first liquid adding inlet is disposed obliquely and the guiding surface is a side surface of the first liquid adding inlet.
7. A sheath flow impedance count device comprising:
a counter assembly comprising a counter, a waste liquid isolation chamber, a rear sheath isolation chamber, and a pressure balance tube; wherein
the counter has a front cell, a rear cell, a rear sheath liquid inlet, and a waste liquid outlet;
the front cell and rear cell are in communication through a counting hole;
the rear sheath liquid inlet and the waste liquid outlet are in communication with the rear cell;
the rear sheath isolation chamber and the rear sheath liquid inlet are connected with each other;
the waste liquid isolation chamber is connected with the waste liquid outlet;
the pressure balance tube is connected with the rear sheath isolation chamber and the waste liquid isolation chamber; and
the pressure balance tube has a pressure balance controller; and
a rear sheath adding tube, a front sheath adding tube, a rear sheath waste liquid discharging tube, and a front sheath waste liquid discharging tube, the front sheath adding tube and the front sheath waste liquid discharging tube being connected to the front cell of the counter assembly, the rear sheath isolation chamber being connected to the rear sheath adding tube and the rear cell of the counter assembly, and the rear sheath waste liquid discharging tube being connected to the rear cell of the counter assembly.
8. The device according to claim 7 , further comprising a first container and a second container, wherein the front sheath adding tube and the rear sheath adding tube are connected to the first container, and the front sheath waste liquid discharging tube and the rear sheath waste liquid discharging tube are connected to the second container, the first container having a positive pressure, and the second container having a negative pressure.
9. The device according to claim 8 , further comprising a shielding box, wherein the counter assembly is located inside of the shielding box; at least one of the rear sheath adding tube, the front sheath adding tube, the rear sheath waste liquid discharging tube, and the front sheath waste liquid discharging tube has an internal tube inside of the shielding box and an external tube outside of the shielding box; and the internal tube and the external tube are connected with each other through a shielding connector.
10. A flow cytometer analyzer comprising:
a counter assembly comprising a counter, a waste liquid isolation chamber, a rear sheath isolation chamber, and a pressure balance tube; wherein
the counter has a front cell, a rear cell, a rear sheath liquid inlet, and a waste liquid outlet;
the front cell and rear cell are in communication through a counting hole;
the rear sheath liquid inlet and the waste liquid outlet are in communication with the rear cell;
the rear sheath isolation chamber and the rear sheath liquid inlet are connected with each other;
the waste liquid isolation chamber is connected with the waste liquid outlet;
the pressure balance tube is connected with the rear sheath isolation chamber and the waste liquid isolation chamber; and
the pressure balance tube has a pressure balance controller.