Heterologous expression of neisserial proteins
View Patent ↗Alternative and improved approaches to the heterologous expression of the proteins of Neisseria meningitidis and Neisseria gonorrhoeae . These approaches typically affect the level of expression, the ease of purification, the cellular localization, and/or the immunological properties of the expressed protein.
1. A composition comprising a hybrid protein comprising a Neisserial 287 protein and a Neisserial 953 protein, wherein the hybrid protein comprises an amino acid sequence having at least eighty percent identity to SEQ ID NO: 91 or SEQ ID NO: 97.
2. The composition of claim 1 , wherein the Neisserial 287 protein and a Neisserial 953 protein are joined by a linker peptide.
3. The composition of claim 2 , wherein the linker peptide is a poly-glycine linker.
4. The composition of claim 1 , wherein the Neisserial 287 protein is a ΔG form.
5. The composition of claim 1 , further comprising a pharmaceutically acceptable carrier.