Use of gingival fibroblasts for vascular cell therapy
View Patent ↗The invention relates to the use of gingival fibroblasts for obtaining a cellular composition for treating arterial-remodelling pathology, for example an aneurysm, post-cryoplasty stenosis and restenosis, an aortic dissection or atherosclerosis.
1. A method for treating an arterial-remodeling pathology, wherein said method comprises implanting gingival fibroblasts into the wall of a lesioned artery, and wherein said pathology is atherosclerosis.
2. The method of claim 1 , wherein said fibroblasts are derived from gingival tissue taken beforehand from the individual for whom the treatment is intended.
3. The method of claim 2 , wherein said fibroblasts are cultured for at least 14 days prior to implantation.
4. The method of claim 3 , wherein said fibroblasts are cultured for 14 to 70 days prior to implantation.
5. The method of claim 1 , wherein said fibroblasts are injected into the adventica or periadventitial tissue.
6. The method of claim 1 , wherein at least 105 gingival fibroblasts are implanted.
7. The method of claim 1 , wherein implanting gingival fibroblasts comprises implanting a composition that includes gingival fibroblasts in combination with decorin or hyaluronic acid.
8. The method of claim 1 , wherein said fibroblasts are labeled with anionic nanoparticles.
9. The method of claim 1 , wherein the lesioned artery has not been subject to angioplasty prior to implantation of said fibroblasts.
10. A method for treating an arterial-remodeling pathology, said pathology being atherosclerosis, comprising obtaining fibroblasts from gingival tissue taken from an individual, culturing said gingival fibroblasts, and implanting said cultured gingival fibroblasts into the wall of a lesioned artery of said individual.
11. The method of claim 10 , wherein said gingival fibroblasts are cultured for at least 14 days prior to implantation.
12. The method of claim 11 , wherein said gingival fibroblasts are cultured for 14 to 70 days prior to implantation.
13. The method of claim 10 , wherein said gingival fibroblasts are injected into the adventica or periadventitial tissue.
14. The method of claim 10 , wherein at least 105 gingival fibroblasts are implanted.
15. The method of claim 10 , wherein implanting gingival fibroblasts comprises implanting a composition that includes gingival fibroblasts in combination with decorin or hyaluronic acid.
16. The method of claim 10 , further comprising labeling said gingival fibroblasts with anionic nanoparticles prior to implantation.
17. The method of claim 10 , wherein the lesioned artery has not been subject to angioplasty prior to implantation of said gingival fibroblasts.
18. A method for treating an arterial-remodeling pathology, said pathology being atherosclerosis, comprising obtaining fibroblasts from gingival tissue taken from an individual, culturing said gingival fibroblasts, and implanting said cultured gingival fibroblasts into the wall of a lesioned artery of said individual, wherein said individual is suffering from an arterial-remodeling pathology involving increased activity of MMP9.