Biochip sensor
Small and extremely small molecules and ions or atoms may be detected with the novel device with exceptional sensitivity. The detection is implemented in a simple manner by the known acoustic resonator FBAR or by means of other technologies that measure the physical properties of the filled layer. The permeability of substances (e.g. active ingredients) through membranes such as cell membranes, lipid bilayers, and cell walls can be examined by combining a sensor with the reservoir and the membrane.
1. A biochip, comprising:
a sensor and a test panel disposed on said sensor;
said sensor being an acoustic resonance sensor with a first electrode disposed on a substrate, a piezoelectric layer disposed on said first electrode, and a second electrode disposed on said piezoelectric layer; and;
said test panel being disposed on said second electrode and having a reservoir formed of an absorbent layer and a membrane on top of said absorbent layer, said reservoir being a multilayer polymer and said membrane being at least one membrane selected from the group consisting of a lipid bilayer, a cell, a cell wall, and a cell membrane;
wherein a thickness of said reservoir and said membrane is greater than a penetration depth of an acoustic wave; and
wherein said sensor is configured to measure a permeability of a substance to be detected through said membrane.
2. The biochip according to claim 1 , wherein said reservoir comprises a porous material.
3. The biochip according to claim 1 , wherein a permeability of said membrane is adapted to the substance to be detected.
4. The biochip according to claim 1 , wherein said reservoir is made of a material selected from the group consisting of polymers, gels, porous ceramic materials and porous metallic materials.
5. The biochip according to claim 1 , wherein said sensor is at least one sensor selected from the group consisting of acoustic sensors, optical sensors, magnetic sensors, and mechanical sensors.
6. The biochip according to claim 1 , wherein said membrane on said reservoir is composed directly of cells or lipid bilayers.
7. The biochip according to claim 1 , which comprises proteins provided within said membrane for influencing a permeability of said membrane.
8. The biochip according to claim 1 , wherein said sensor is configured to react to a change in an optical property of said test panel.
9. The biochip according to claim 1 , wherein said reservoir comprises a polyelectrolyte multilayer polymer.
10. The biochip according to claim 9 , wherein said polyelectrolyte multilayer polymer contains polyelectrolytes selected from the group consisting of polyetherimide (PEI), polyallylamine (PAH), polyglutamic acid (PGA), and polystyrol sulfonate (PSS).
11. The biochip according to claim 1 , wherein said reservoir is a dual reservoir including an unfilled reservoir portion and a filled reservoir portion adjacent said unfilled reservoir portion, wherein said filled reservoir portion is filled with the substance to be detected and said unfilled reservoir portion is not filled with the substance to be detected, and wherein said sensor is configured to detect an acoustic wave traversing said unfilled reservoir portion and an acoustic wave traversing said filled reservoir portion.