Engineered CO2 fixing microorganisms producing carbon-based products of interest
The present disclosure identifies pathways and mechanisms to confer production of carbon-based products of interest such as ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof in photoautotrophic organisms such that these organisms efficiently convert carbon dioxide and light into carbon-based products of interest, and in particular the use of such organisms for the commercial production of ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof.
1. An engineered photosynthetic microbe for biofuel production, wherein said photosynthetic microbe comprises a heterologous pyruvate decarboxylase, a heterologous NADPH-dependent alcohol dehydrogenase, and a heterologous catalase.
2. The engineered photosynthetic microbe of claim 1 , wherein the heterologous catalase is Synechocystis sp. PCC 6803 katG.
3. The engineered photosynthetic microbe of claim 2 , wherein the heterologous pyruvate decarboxylase is Zymomonas mobilis pyruvate decarboxylase.
4. The engineered photosynthetic microbe of claim 2 or 3 , wherein the heterologous alcohol dehydrogenase is Zymomonas mobilis adhA.
5. The engineered photosynthetic microbe of claim 2 or 3 , wherein the heterologous alcohol dehydrogenase is Moorella sp. HUC22-1 adhA.
6. The engineered photosynthetic microbe of claim 1 , wherein the photosynthetic microbe comprises heterologous Synechocystis sp. PCC6803 katG, a heterologous Zymomonas mobilis pyruvate decarboxylase (DD 161175), and a heterologous Zymomonas mobilis adhII.
7. The engineered photosynthetic microbe of claim 1 , wherein the photosynthetic microbe comprises a heterologous Synechocystis sp. PCC6803 katG, a heterologous Zymomonas mobilis pyruvate decarboxylase (DD 161175), and a heterologous Moorella sp. HUC22-1 adhA.
8. The engineered photosynthetic microbe of claim 1 wherein said photosynthetic microbe is a cyanobacterium.
9. The engineered photosynthetic microbe of claim 8 , wherein said cynanobacterium is a Synechococcus species.
10. The engineered photosynthetic microbe of claim 1 , wherein the NADPH-dependent alcohol dehydrogenase is an enzyme that has EC 1.1.1.2.
11. The engineered photosynthetic microbe of claim 1 , wherein the NADPH-dependent alcohol dehydrogenase comprises an amino acid sequence that is at least 95% identical to the amino acid sequence shown in SEQ ID NO:2.
12. The engineered photosynthetic microbe of claim 1 , wherein the NADPH-dependent alcohol dehydrogenase comprises the amino acid sequence shown in SEQ ID NO:2.