Porous-wall hollow glass microspheres as carriers for biomolecules
View Patent ↗The present invention includes compositions of porous-wall hollow glass microspheres and one or more biomolecules, wherein the one or more biomolecules are positioned within a void location within the hollow glass microsphere, and the use of such compositions for the diagnostic and/or therapeutic delivery of biomolecules.
1. A hollow microsphere comprising:
a glass wall surrounding an internal volume to form a glass microsphere having a diameter of about 10 microns to about 100 microns, wherein the glass wall comprises an external surface and an internal surface, and
dextran-gated channels having a diameter of about 1 nm to about 100 nm connecting the external surface and the internal volume, wherein the internal volume comprises a biopolymer and wherein the dextran-gated channels passively regulate passage of the biopolymer out of the hollow microsphere.
2. The hollow microsphere of claim 1 , wherein the hollow microsphere has a diameter ranging from about 20 microns to about 50 microns.
3. The hollow microsphere of claim 1 , wherein the channels have a diameter of about 10 nanometers (nm).
4. The hollow microsphere of claim 1 , wherein the biopolymer is selected from the group consisting of an antibody or antigen-binding fragment or derivative thereof, a protein, a nucleic acid, a hormone, a growth factor, a virus, and combinations thereof.
5. The hollow microsphere of claim 1 , wherein the biopolymer comprises a single chain Fv.
6. The hollow microsphere of claim 1 , wherein the biopolymer comprises a short interfering RNA (siRNA) or an anti-sense RNA.
7. The hollow microsphere of claim 1 , wherein the biopolymer has a size of about 3 nanometers to about 8 nanometers.
8. The hollow microsphere of claim 1 , wherein the channels have a diameter of a size of about the same size of the biopolymer.
9. The hollow microsphere of claim 1 , wherein the channels have a diameter of a size greater than the size of the biopolymer.
10. The hollow microsphere of claim 1 , wherein the gated channels comprise a gating agent having a diameter about the same as the of the channels.
11. The hollow microsphere of claim 10 , wherein the gating agent promotes retention of the biopolymer within the internal volume of the hollow microsphere.
12. A composition comprising the hollow microsphere of claim 1 .
13. A method of providing a biopolymer to a subject, the method comprising administering a composition comprising the hollow microsphere of claim 1 to the subject.
14. The method of claim 13 wherein the composition is administered intratumorally.
15. The method of claim 13 wherein the composition is administered via the hepatic artery.