Delivery vehicle for probiotic bacteria comprising a dry matrix of polysaccharides, saccharides and polyols in a glass form and methods of making same
The disclosure relates to a solid glass matrix of polysaccharide, saccharides and polyols as delivery vehicles for preservation and post gastric administration of a probiotic. The delivery vehicle is capable of releasing the probiotic at their site of action. The present invention further includes methods of making and using the solid glass matrix delivery vehicle of the invention.
1. A method for preparing a preservation mixture, comprising:
(a) gelling a mixture comprising a polysaccharide, a disaccharide, a sugar alcohol and a probiotic bacterium at a low temperature, whereby a gel mixture is made,
(b) slicing the gel mixture into small pieces, and
(c) drying the small pieces under vacuum under conditions suitable for glass formation, whereby the preservation mixture is prepared.
2. The method of claim 1 , further comprising cross-linking the polysaccharide in step (a).
3. The method of claim 1 , wherein the probiotic bacteria are selected from the group consisting of live Lactobacillus, Bifidobacterium, Enterococcus, Propionobacterium, Bacillus and Streptococcus.
4. The method of claim 1 , wherein the polysaccharide is selected from the group consisting of starch, non-digestible starch, pectin, inulin, xanthan gum, alginate, alginic acid, chitosan, carrageenan, carboxymethyl cellulose, methyl cellulose, guar gum, gum arabic, locust bean gum and combinations thereof.
5. The method of claim 1 , wherein the sugar alcohol is selected from the sugar alcohols group consisting of mannitol, sorbitol, xylitol, maltitol, lactitol and isomalt.
6. The method of claim 1 , wherein the disaccharide is sucrose.
7. The method of claim 1 , further comprising
(d) maintaining the preservation mixture of step (c) at 40° C.,
(e) applying to the preservation mixture from step (d) a first vacuum up to 2,500 mTORR for a first period of time; and
(f) applying to the preservation mixture from step (e) a second vacuum at 100 mTORR for a second period of time.
8. The method of claim 7 , wherein the pressure during the first period of time is reduced to 2,500 mTORR.
9. The method of claim 8 , wherein the pressure during the second period of time is reduced to less than 100 mTORR.
10. The method of claim 8 , wherein the pressure during the first period of time is reduced in increments to the pressure during the second period of time.
11. The method of claim 8 , wherein the temperature in the dryer is maintained at 10-20° C. during the first period of time.
12. The method of claim 8 , wherein the temperature in the dryer is maintained at 40-50° C. during the second period of time.
13. The method of claim 8 , wherein the first period of time lasts from 12 to 16 hours.
14. The method of claim 8 , wherein the second period of time stage lasts from 12 to 48 hours.