Methods for Reducing Gluconoylation of Proteins
The present invention relates to methods of preventing glyconoylation of polypeptides produced in micororganisms.
1 .- 15 . (canceled)
16 . A method for preventing gluconoylation of polypeptides expressed in a microorganism, comprising introducing DNA into the microorganism, wherein the DNA encodes a polypeptide that demonstrates 6-phosphogluconolactonase activity.
17 . The method of claim 16 , wherein the microorganism is E. coli.
18 . The method of claim 16 , wherein the DNA comprises DNA encoding a 6-phosphogluconolactonase enzyme.
19 . The method of claim 18 , wherein the DNA encoding the 6-phosphogluconolactonase enzyme has at least 70% sequence identity to DNA encoding 6-phosphogluconolactonase from Pseudomonas.
20 . The method of claim 19 , wherein the Pseudomonas is P. aeruginosa.
21 . The method of claim 18 , wherein the DNA encoding the 6-phosphogluconolactonase enzyme has at least 90% sequence identity to SEQ ID NO:7.
22 . The method of claim 18 , wherein the DNA encoding the 6-phosphogluconolactonase enzyme has at least 95% sequence identity to SEQ ID NO:7.
23 . The method of claim 18 , wherein the 6-phosphogluconolactonase enzyme has an amino acid sequence having at least 90% sequence identity to SEQ ID NO:8.
24 . The method of claim 18 , wherein the 6-phosphogluconolactonase enzyme has an amino acid sequence having at least 95% sequence identity to SEQ ID NO:8.
25 . The method of claim 18 , wherein the DNA encoding the 6-phosphogluconolactonase enzyme comprises the sequence set forth in SEQ ID NO:7.
26 . The method of claim 16 , wherein the DNA is recombinant DNA.
27 . The method of claim 16 , wherein the DNA is transformed into the genomic DNA of the microorganism.
28 .- 59 . (canceled)