Tropoelastin derivatives
View Patent ↗The invention relates to derivatives of tropoelastin and variants of those derivatives. The invention further provides expression products and hybrid molecules of the derivatives and variants of the invention. The invention further provides methods for the production of the derivatives, variants, expression products and hybrid molecules. Further provided are formulations, cross-linked structures and implants comprising the derivatives, variants, expression products and hybrid molecules of the invention. Further provided are uses of the derivatives, variants, expression products and hybrid molecules of the invention.
1. A method for forming an implant having a pre-defined glycosaminoglycan (GAG) binding activity comprising:
(a) selecting tropoelastin monomers;
(b) forming a composition with the selected monomers; and
(c) cross linking the monomers in the composition in the presence of a mould for forming an implant wherein an implant having GAG binding activity is formed when a tropoelastin monomer containing tropoelastin domain 26A is selected, thereby forming an implant having a pre-defined glycosaminoglycan (GAG) binding activity and wherein the implant comprises a tropoelastin selected from the group consisting of SHELδmodified (SEQ ID NO: 5), SHELgamma (SEQ ID NO: 9), SHEL31-36 (SEQ ID NO: 10), SHEL32-36 (SEQ ID NO: 11 and SHELδ26A SEQ ID NO: 3).
2. The method of claim 1 wherein the tropoelastin is cross-linked by cross-linking of lysine or glutamic acid side chains in tropoelastin.
3. The method of claim 1 wherein the mould takes the form of a flat surface for forming an implant into sheet form.
4. The method of claim 1 wherein the GAG binding activity of the cross-linked tropoelastin obtained from the composition is adjusted based on a comparison of a SHEL to SHELd26A amount in the composition.