Fluid stream imaging apparatus
View Patent ↗A fluid stream imaging apparatus having either optics for manipulating the aspect ratio or sensing elements configured for manipulating the aspect ratio of an image of the fluid stream. This application may also relate to a system for acquiring images of a portion of a fluid stream at high speeds for image processing to measure and predict droplet delays for individual forming particles.
1. A flow cytometer comprising:
a. a fluid delivery system having a nozzle for producing a fluid stream, the fluid delivery system having an oscillator for perturbing the fluid stream into droplets at a break off point downstream of the nozzle;
b. an excitation energy source for interrogating the fluid stream at an inspection zone;
c. an optical system for manipulating the aspect ratio of an image of the fluid stream; and
d. a sensing element for sensing the manipulated image of the fluid stream.
2. The flow cytometer of claim 1 wherein the optical system further comprises a first optical element and a second optical element for manipulating the aspect ratio of the image of the fluid stream.
3. The flow cytometer of claim 1 wherein one or more of the optical elements comprise refractive elements for altering the aspect ratio of the image of the fluid stream.
4. The flow cytometer of claim 3 wherein one or more of the refractive elements comprise a lens.
5. The flow cytometer of claim 4 wherein the one or more lenses comprise a first cylindrical lens for manipulating the ratio of the image of the fluid stream in a first axis.
6. The flow cytometer of claim 5 wherein the one or more lenses comprise a second cylindrical lens for manipulating the ratio of the image of the fluid stream in a second axis which is orthogonal to the first axis.
7. The flow cytometer of claim 6 wherein the second lens increases the aspect ratio in the second axis.
8. The flow cytometer of claim 6 wherein the ratio of the manipulated first axis to the manipulated second axis is 10 to 1.
9. The flow cytometer of claim 6 wherein the length of the image of the fluid stream is compressed and wherein width of the image of the fluid stream is expanded.
10. The flow cytometer of claim 5 wherein the first lens reduces the aspect ratio in the first axis.
11. The flow cytometer of claim 1 wherein the optical elements further comprise reflective elements for altering the aspect ratio of the image of the fluid stream.
12. The flow cytometer of claim 11 wherein the reflective elements comprises one or more mirrors.
13. The flow cytometer of claim 12 wherein the one or more mirrors comprise a first mirror for manipulating the ratio of the image of the fluid stream in a first axis.
14. The flow cytometer of claim 13 wherein the one or more mirrors comprise a second mirror for manipulating the ratio of the image of the fluid stream in a second axis which is orthogonal to the first axis.
15. The flow cytometer of claim 1 wherein the optical elements further comprise diffractive elements for altering the aspect ratio of the image of the fluid stream.
16. The flow cytometer of claim 1 further comprising an illumination source for illuminating the stream.
17. The flow cytometer of claim 16 wherein the illumination source comprises a strobe.
18. The flow cytometer of claim 1 wherein the manipulated image of the fluid stream includes the inspection zone and the break off point in a single image of the fluid stream.
19. The flow cytometer of claim 1 wherein the manipulated image of the fluid stream includes a portion of the nozzle and the break off point in a single image of the fluid stream.
20. The flow cytometer of claim 1 wherein the sensing element is one selected from the group consisting of: a camera, a CCD, CMOS, photodiode array, sensor array, and combinations thereof.
21. The flow cytometer of claim 1 wherein the optical system for the manipulation of the aspect ratio of the fluid stream further comprises an optical element for magnifying the width of the stream by a factor between 3 and 20.
22. The flow cytometer of claim 1 wherein the optical system for the manipulation of the aspect ratio of the fluid stream further comprises an optical element for compressing the length of the fluid stream by a factor between ¼ and 10.
23. The flow cytometer of claim 1 wherein the optics system comprises gradient index optics for manipulating the aspect ratio of the image of the fluid steam differently at different locations.
24. The flow cytometer of claim 23 wherein the aspect ratio of the image of the fluid stream is manipulated at a first aspect ratio at the inspection zone and the image of the fluid stream is manipulated at a second aspect ratio at the break off point.
25. The flow cytometer of claim 24 wherein the aspect ratio is modified in a continuous gradient from the first aspect ratio to the second aspect ratio.
26. The flow cytometer of claim 24 wherein the aspect ratio is modified in at two or more disjointed aspect ratios.