Formation of N-protected bis-3,6-(4-aminoalkyl)-2,5,diketopiperazine
View Patent ↗The disclosed embodiments detail improved methods for the synthesis of diketopiperazines from amino acids. In particular improved methods for the cyclocondensation and purification of N-protected 3,6-(aminoalkyl)-2,5-diketopiperazines from N-protected amino acids. Disclosed embodiments describe methods for the synthesis of 3,6-bis-[N-protected aminoalkyl]-2,5-diketopiperazine comprising heating a mixture of an amino acid in the presence of a catalyst in an organic solvent. The catalyst is selected from the group comprising sulfuric acid, phosphoric acid, p-toluenesulfonic acid, 1-propylphosphonic acid cyclic anhydride, tributyl phosphate, phenyl phosphonic acid and phosphorous pentoxide among others. The solvent is selected from the group comprising: dimethylacetamide, N-methyl-2-pyrrolidone, diglyme, ethyl glyme, proglyme, ethyldiglyme, m-cresol, p-cresol, o-cresol, xylenes, ethylene glycol and phenol among others.
1. A method for the synthesis of 3,6-bis-4-(N-trifluoroacetyl)aminobutyl-2,5- diketopiperazine comprising:
heating a mixture of ε-trifluoroacetyl-L-lysine in the presence of phosphorous pentoxide in N-methyl-2-pyrrolidone, to a temperature of between 150° and 175° C. for between 0.25 and 5 hours, the concentration of phosphorous pentoxide is from 20% to 35% that of the lysine; and quenching the mixture with water.
2. The method of claim 1 , wherein the mixture is heated to a temperature of between 160° and 170° C.
3. The method of claim 1 , wherein the mixture is heated for between 1 and 2 hours.
4. The method of claim 1 , wherein the mixture is cooled prior to quenching.
5. The method of claim 1 , wherein the mixture is heated to a temperature of between 160° and 165° C.
6. The method of claim 1 , wherein the mixture is heated for about 1.5 hours.
7. The method of claim 1 , wherein the concentration of phosphorous pentoxide is from about 20% to 30% that of the lysine.