TISSUE DISSAGGREGATOR DEVICE AND METHODS OF USING THE SAME
The tissue disaggregator device includes a base having a multiple well array. Each of the wells includes a screen embedded into bottom portion of the well. The device removably connects with standardized plate and allows for multiple fold increase in tissue sample preparation.
1 . A tissue disaggregator device comprising:
a base having an array of multiple wells, wherein each of said wells comprises a bottom portion having at least one screen.
2 . The device of claim 1 wherein the base comprise a plastic material.
3 . The device of claim 1 wherein the base comprise a transparent material.
4 . The device of claim 1 wherein the base comprise a chemical resistant material.
5 . The device of claim 1 wherein the base comprise an autoclave safe material.
6 . The device of claim 1 wherein the screen comprise one of steel, mesh, nylon, plastic and rubber material.
7 . The device of claim 6 wherein the screen is embedded into the bottom portion of the well.
8 . The device of claim 1 wherein the base is configured to removably mate with a plate having an array of multiple plate wells.
9 . The device of claim 1 wherein a gap is formed between the screen and the plate well upon the mate.
10 . The device of claim 9 wherein a liquid solution is placed in the well prior to receipt of a tissue.
11 . The device of claim 10 wherein the liquid solution floats in the well and is configured to flow from the screen into the gap and the plate well.
12 . The device of claim 11 wherein the mate allows for tissue cells to flow from the screen to be collected in the gap.
13 . A method comprising:
providing a tissue disaggregator device having a base with an array of multiple wells, wherein said base is removably mated with a plate having an array of multiple plate wells;
pouring a liquid solution into multiple wells of the base, wherein each of said multiple wells comprise a bottom portion having at least one screen to allow for floating of a liquid solution; and
depositing at least one tissue directly onto the screen, wherein the tissue remains viable in the liquid solution.
14 . The method of claim 13 further comprising applying a force onto the tissue to force tissue through the screen thereby disaggregating the tissue structure to produce cells in suspension.
15 . The method of claim 14 wherein the applied force pushes the cells into an area between the screen and the plate well of the plate.