IP Library Granted Patent US 9,364,803
Granted Patent B2
US 9,364,803 · App. 13/371,222 · Granted Jun 14, 2016

Methods for forming mixed droplets

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Quick Facts
Patent No.
US 9,364,803
App. No.
13/371,222
Granted
Jun 14, 2016
Kind
B2
Abstract

The invention generally relates to methods for forming mixed droplets. In certain embodiments, methods of the invention involve forming a droplet, and contacting the droplet with a fluid stream, wherein a portion of the fluid stream integrates with the droplet to form a mixed droplet.

Claims (27)

1. A method for forming a mixed droplet, the method comprising:

forming a droplet of a first fluid;

flowing the droplet of the first fluid in a first channel comprising a drop track thereby flowing the droplet of the first fluid away from center streamline and toward a bolus of a fluid stream; and

contacting the droplet of the first fluid with the bolus of the fluid stream wherein a portion of the bolus segments from the fluid stream and integrates with the droplet of the first fluid to form a mixed droplet.

2. The method according to claim 1 , wherein the fluid stream is delivered via a second channel oriented perpendicular to the first channel.

3. The method according to claim 2 , further comprising applying an electric field to the droplet of the first fluid and the bolus of the fluid stream.

4. The method according to claim 3 , wherein the electric field is a high-frequency electric field.

5. The method according to claim 1 , wherein the droplet of the first fluid is surrounded by an immiscible carrier fluid.

6. The method according to claim 5 , wherein the immiscible carrier fluid is an oil.

7. The method according to claim 6 , wherein the oil comprises a surfactant.

8. The method according to claim 7 , wherein the surfactant is a fluorosurfactant.

9. The method according to claim 1 , wherein the mixed droplet is surrounded by an immiscible carrier fluid.

10. The method of claim 1 , wherein the droplets of the first fluid are monodispersive.

11. The method of claim 1 , wherein the drop track forces the droplet of the first fluid into a lower energy conformation.

12. The method of claim 1 , wherein the bolus protrudes into a first stream comprising the droplet of the first fluid.

13. The method of claim 1 , wherein the drop track has a channel height smaller than the diameter of the droplet.

14. A method for forming a mixed droplet, the method comprising:

forming a droplet of a first fluid surrounded by an immiscible carrier fluid;

flowing the droplet of the first fluid through a first channel comprising a drop track thereby flowing the droplet of the first fluid away from center streamline and toward a bolus of a fluid stream from a second channel; and

contacting the droplet of the first fluid in the first channel with the bolus of the fluid stream from the second channel, wherein a portion of the bolus that segments from the fluid stream integrates with the droplet of the first fluid to form a mixed droplet.

15. The method according to claim 14 , wherein the drop track has a channel height smaller than the diameter of the droplet.

16. The method according to claim 15 , wherein the first and second channels are oriented perpendicular to each other.

17. The method according to claim 14 , wherein the contacting step occurs in the presence of an electric field, and the electric field is a high-frequency electric field.

18. The method according to claim 14 , wherein the mixed droplet is surrounded by an immiscible carrier fluid.

19. The method according to claim 18 , wherein the an immiscible carrier fluid is an oil.

20. The method according to claim 19 , wherein the oil comprises a surfactant.

21. The method according to claim 20 , wherein the surfactant is a fluorosurfactant.

Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 7, 2019
From: RAINDANCE TECHNOLOGIES, INC.
To: BIO-RAD LABORATORIES, INC.
Reel/Frame 049109/0498 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 15, 2012
From: LARSON, JONATHAN WILLIAM, MR.
To: RAINDANCE TECHNOLOGIES, INC.
Reel/Frame 028209/0968 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 30, 2012
From: YURKOVETSKY, YEVGENY; LINK, DARREN ROY
To: RAINDANCE TECHNOLOGIES, INC.
Reel/Frame 028126/0668 →