Microorganisms and methods for conversion of syngas and other carbon sources to useful products
View Patent ↗A non-naturally occurring microbial organism having an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway includes at least one exogenous nucleic acid encoding an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway enzyme expressed in a sufficient amount to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol. The aforementioned organisms are cultured to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol.
1. A non-naturally occurring microbial organism comprising:
(i) a 1,4-butanediol pathway, wherein said microbial organism comprises at least one exogenous nucleic acid encoding a 1,4-butanediol pathway enzyme expressed in a sufficient amount to produce 1,4-butanediol, wherein said 1,4-butanediol pathway enzyme is selected from the group consisting of an acetoacetyl-CoA thiolase, a 3-hydroxybutyryl-CoA dehydrogenase, a crotonase, a crotonyl-CoA hydratase, a 4-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl-CoA reductase (aldehyde forming), and a 1,4-butanediol dehydrogenase; and
(ii) an acetyl-CoA pathway, wherein said microbial organism comprises at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme expressed in a sufficient amount to produce acetyl-CoA, wherein said acetyl-CoA pathway enzyme is selected from the group consisting of an acetyl-CoA synthase, a formate dehydrogenase, a formyltetrahydrofolate synthetase, a methenyltetrahydrofolate cyclohydrolase, a methylenetetrahydrofolate dehydrogenase, and a methylenetetrahydrofolate reductase.
2. The organism of claim 1 , wherein said formate dehydrogenase is encoded by a gene selected from the group consisting of moth — 2312, moth — 2313, moth — 2314, sfum — 2703, sfum — 2704, sfum — 2705, sfum — 2706, chy — 0731, chy — 0732, and chy — 0733.
3. The organism of claim 1 , wherein said formyltetrahydrofolate synthetase is encoded by a gene selected from the group consisting of moth — 0109, chy — 2385, and fhs.
4. The organism of claim 1 , wherein said methenyltetrahydrofolate cyclohydrolase is encoded by a gene selected from the group consisting of moth — 1516, folD, and chy — 1878.
5. The organism of claim 1 , wherein said methylenetetrahydrofolate dehydrogenase is encoded by a gene selected from the group consisting of moth — 1516, folD, and chy — 1878.
6. The organism of claim 1 , wherein said methylenetetrahydrofolate reductase is encoded by a gene selected from the group consisting of moth — 1191, metF, and chy — 1233.
7. The organism of claim 1 , wherein said organism utilizes a feedstock selected from the group consisting of: 1) CO, 2) CO 2 and H 2 , 3) CO and CO 2 , 4) synthesis gas comprising CO and H 2 , 5) synthesis gas comprising CO, CO 2 , and H 2 , and 6) one or more carbohydrates.
8. A method for producing 1,4-butanediol, comprising culturing the non-naturally occurring microbial organism of claim 1 .