Device and method for multi-photon fluorescence microscopy for obtaining information from biological tissue
A device for multi-photon fluorescence microscopy for obtaining information from biological tissue is provided. The device comprising a laser unit for generating an excitation radiation, an optical unit which is formed to focus the excitation radiation for generating an optical signal at different locations in or on an object to be examined, and a detector module for detecting the optical signal from the region of the object. The optical unit for generating the optical signal at different locations in or on the object is movable in at least one direction relative to the object.
1. A device for multi-photon fluorescence microscopy for obtaining information from biological tissue, comprising:
a laser unit for generating an excitation radiation,
an optical unit which is formed to focus the excitation radiation for generating an optical signal at different locations in or on an object to be examined,
a control and processing unit,
a patient module connected to the control and the processing unit, which for examining the object can be placed relative to the object, wherein the optical is part of the patient module, and
a detector module for detecting the optical signal from the region of the object,
wherein the optical unit for generating the optical signal at different locations in or on the object is movable within a housing of the patient module in at least one direction relative to the object,
wherein the laser unit is part of the control and processing unit and an optical fiber connects the laser unit with the patient module for transmitting the excitation radiation towards the optical unit,
wherein the laser unit generates an excitation radiation with a first wavelength to transmit the excitation radiation through the optical fiber, and a frequency doubler is arranged in the patient module for halving the wavelength of the excitation radiation prior to the optical unit,
wherein from the received optical signal is a sectional image of the object is generated pixel by pixel at different locations of the object,
wherein for generating the sectional image pixel by pixel the object is exposed to the excitation radiation in a triggered manner, and
wherein the pixel size of the sectional image pixel by pixel is determined in horizontal direction by the focus of the excitation radiation and by adjusting the triggering, and in vertical direction by the waist length of the focused excitation radiation,
wherein the pixel size is adjustable by a beam expansion of the excitation radiation and by adjusting the triggering.
2. The device according to claim 1 ,
wherein during a movement of the optical unit for generating the optical signal the angular position of the optical axis of the excitation radiation falling onto the object is not changed.
3. The device according to claim 1 or 2 , wherein the optical unit is movable in horizontal direction and/or in vertical direction relative to a surface of the object facing the optical unit.
4. The device according to claim 3 , wherein the optical unit includes an objective for focusing the excitation radiation at a location in or on the object, wherein the objective is movable in vertical direction relative to the surface of the object.
5. The device according to claim 1 , wherein for generating a vertical sectional image pixel by pixel the optical unit is at least partly continuously movable in horizontal direction and/or in vertical direction relative to the object.
6. The device according to claim 1 , wherein the patient module is connected with the control and processing unit via a supporting arm, which for examining the object can be placed relative to the object.
7. The device according to claim 6 , wherein the optical unit is movable independent of the optical fiber for transmitting the excitation radiation.
8. The device according to claim 1 , wherein the optical unit is formed for collecting the optical signal.
9. The device according to claim 8 , wherein via an optical fiber the optical unit is connected with the detector module for transmitting the recorded optical signal to the detector module.
10. The device according to claim 9 , wherein the optical fiber for transmitting the recorded optical signal to the detector module differs from an optical fiber provided for transmitting the excitation radiation.
11. The device according to claim 8 , characterized wherein the detector module is formed to evaluate the different signal components for imaging and/or for the spectroscopic analysis.
12. The device according to any of the preceding claim 1 , wherein the detector module is formed to split up the received optical signal into a plurality of different signal components in different wavelength ranges and for this purpose includes one or more dichroic filter elements.
13. A method for multi-photon fluorescence microscopy for obtaining information from biological tissue, in which
a laser unit generates an excitation radiation,
an optical unit focuses the excitation radiation for generating an optical signal at different locations in or on an object to be examined,
a control and processing unit,
a patient module connected to the control and the processing unit, which for examining the object can be placed relative to the object, wherein the optical is part of the patient module, and
a detector module detects the optical signal from the region of the object,
wherein the optical unit for generating the optical signal in or on the object is moved within a housing of the patient module in at least one direction relative to the object,
wherein the laser unit is part of the control and processing unit and an optical fiber connects the laser unit with the patient module for transmitting the excitation radiation towards the optical unit, and
wherein the laser unit generates an excitation radiation with a first wavelength to transmit the excitation radiation through the optical fiber, and a frequency doubler is arranged in the patient module for halving the wavelength of the excitation radiation prior to the optical unit,
wherein from the received optical signal is a sectional image of the object is generated pixel by pixel at different locations of the object,
wherein for generating the sectional image pixel by pixel the object is exposed to the excitation radiation in a triggered manner, and
wherein the pixel size of the sectional image pixel by pixel is determined in horizontal direction by the focus of the excitation radiation and by adjusting the triggering, and in vertical direction by the waist length of the focused excitation radiation,
wherein the pixel size is adjustable by a beam expansion of the excitation radiation and by adjusting the triggering.
14. The method according to claim 13 at wherein the optical unit is moved exclusively in horizontal direction to the surface of the object and the optical signal is integrated in vertical direction.