Tomato plants resulting from the introgression of a trait from
View Patent ↗The present invention provides a method for the production of S. lycopersicum plants having an average sympodial index of 2 and producing red-colored fruits comprising crossing a plant of S. lycopersicum capable of producing red-colored fruits, with a plant of a Solanum spp. having an average sympodial index of 2, collecting the seeds resulting from the cross, regenerating the seeds into plants, providing one or more backcross generations, selfing the backross plants, growing the selfed seed into plants, and identifying and selecting plants having an average sympodial index of 2 and producing red-colored fruits.
1. A method for the production of a Solanum lycopersicum plant having an average sympodial index of between 1.8 and 2.2 and producing red-colored fruits, said method comprising:
a) crossing a plant of a recipient breeding line of S. lycopersicum capable of producing red-colored fruits, with a plant of a donor line of S. pennellii , whereby plants of said donor line have an average sympodial index of between 1.8 and 2.2 and produce green tomatoes;
b) collecting the seeds resulting from the cross in step (a),
c) regenerating the seeds into plants;
d) providing one or more backcross generations by crossing the plants of step (c) or optionally selfed offspring thereof with one or more plants of said recipient breeding line of S. lycopersicum to provide backcross plants;
e) selfing plants of step (d) and growing the selfed seed into plants;
f) optionally repeating said steps of backcrossing and selfing of steps (d) or (e);
g) identifying and selecting from the plants grown in step (c), (e) or (f) plants having an average sympodial index of between 1.8 and 2.2 and producing red-colored fruits, wherein the step of identifying and selecting a plant having an average sympodial index of between 1.8 and 2.2 is performed by marker-assisted selection comprising the use of a marker linked to a SP3D gene from the family of self-pruning genes of S. pennellii , and the step of identifying and selecting a plant producing red colored fruits comprises use of a marker linked to phytoene synthase PSY1 of S. lycopersicum.
2. Method according to claim 1 , wherein said Solanum lycopersicum plant having an average sympodial index of between 1.8 and 2.2 and producing red-colored fruits is an indeterminate plant.
3. Method according to claim 1 , wherein said donor line of a Solanum spp. having an average sympodial index of between 1.8 and 2.2 is the wild tomato species S. pennellii LA716.
4. The method according to claim 1 , wherein the marker linked to the SP3D gene from the family of self-pruning genes is provided by the presence of the sequence provided in SEQ ID NO:9.
5. The method according to claim 1 , wherein the marker linked to the SP3D gene from the family of self-pruning genes is provided by the presence of the sequence provided in SEQ ID NO:14.
6. The method according to claim 1 , wherein said marker linked to a SP3D gene consists of a primer having SEQ ID NO:16 and a primer having SEQ ID NO:17, in combination with a restriction enzyme EcoR V, whereby a banding pattern of one band of 742 bp is indicative for a plant having an average sympodial index of between 1.8 and 2.2.
7. The method according to claim 1 , wherein the marker linked to phytoene synthase PSY1 is provided by the presence of the sequence provided in SEQ ID NO:6.
8. The method according to claim 1 , wherein the marker linked to phytoene synthase PSY1 is provided by the presence of the sequence provided in SEQ ID NO:11.
9. The method according to claim 1 , wherein said marker linked to phytoene synthase PSY1 consists of a primer having SEQ ID NO:18 and a primer having SEQ ID NO:19, in combination with the restriction enzyme Bsh1236 I, whereby a banding pattern of two bands of 463 bp and 482 bp is indicative of a plant producing red colored fruits.