Devices and methods for electroosmotic transport of non-polar solvents
View Patent ↗A microdevice for supporting a flowing nonpolar fluid is disclosed. The microdevice includes a substrate that is at least partially coated by one or more amphiphilic layers. Methods for using the device in biological and chemical assays are also disclosed.
1. A microfluidic device comprising: at least one fluid channel and at least one pair of electrodes configured to provide an electric field in the at least one channel, wherein the interior of the at least one channel is at least partially coated by an amphiphile forming a lipid bilayer, the lipid bilayer comprising an intercalated non-polar solvent and the interior of at least one channel comprises two or more materials selected from the group consisting of: mica, glass, quartz, platinum, stainless steel, copper, aluminum, nickel, gold, titanium, ceramic, diamond, silicon, silicon nitride, silicone, high-density polyethylene, polyethylene terephthalate, polydimethylsiloxane, polymethyl-methacrylate, polystyrene, cellulose acetate, polyimide, and polycarbonate.
2. The device of claim 1 , wherein the lipid bilayer comprises a phospholipid, sphingolipid, or glycolipid.
3. The device of claim 2 , wherein the phospholipid is selected from the group consisting of: phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, and phosphatidylglycerol.
4. The device of claim 1 , wherein the lipid bilayer has a fatty acyl chain length which defines a volume of an interior hydrophobic region of the channel.
5. The device of claim 4 , wherein the fatty acyl chain length is about 2 carbons to about 24 carbons.
6. The device of claim 1 , wherein the interior of the at least one channel is completely covered by the lipid bilayer.
7. The device of claim 1 , wherein the at least one channel has a diameter of about 0.1 μM to about 500 μm.
8. The device of claim 1 further comprising a voltage controller in electrical contact with the at least one pair of electrodes.
9. The device of claim 1 further comprising one or more input ports and one or more output ports in fluid communication with the at least one channel.
10. A method of circulating or conducting a non-polar fluid, the method comprising:
adding a non-polar fluid to a microfluidic device comprising at least one fluid channel and at least one pair of electrodes configured to provide an electric field in the at least one channel, wherein the interior of the at least one channel is at least partially coated by an amphiphile forming a lipid bilayer, the lipid bilayer comprising an intercalated non-polar solvent and the interior of at least one channel comprises two or more materials selected from the group consisting of: mica, glass, quartz, platinum, stainless steel, copper, aluminum, nickel, gold, titanium, ceramic, diamond, silicon, silicon nitride, silicone, high-density polyethylene, polyethylene terephthalate, polydimethylsiloxane, polymethyl-methacrylate, polystyrene, cellulose acetate, polyimide, and polycarbonate; and
applying an electric field to the at least one fluid channel to generate an electro-osmotic flow.
11. The method of claim 10 , wherein the non-polar fluid is selected from the group consisting of: propane, butane, pentane, hexane, heptane, octane, nonane, decane, cyclopentane, cyclohexane, benzene, toluene, xylene, squalene, diethyl ether, diisopropylether, ethylacetate, 2-butanone, carbon tetrachloride, chloroform, methylene chloride, tetrachloroethane, trichlorethane, dichloroethane, and ethyl acetate.
12. The method of claim 11 , wherein the lipid bilayer forms a hydrophobic space in the interior of the channel.
13. The method of claim 11 , wherein the lipid bilayer comprises a phospholipid, sphingolipid, or glycolipid.
14. The method of claim 13 , wherein the phospholipid is selected from the group consisting of: phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, and phosphatidylglycerol.
15. The method of claim 11 , wherein the one or more lipid layers has a fatty acyl chain length of about 2 carbons to about 24 carbons which defines the volume of an interior hydrophobic region of the channel.