Methods for treating inhalation injury
The invention is directed to methods for treating inhalation injuries. Such methods utilize novel cell compositions such extraembryonic cytokine secreting (ECS) cells and Amnion-derived Multipotent Progenitor (AMP) cells and novel cellular factor-containing solution compositions such as extraembryonic cell-derived cellular cytokine solution, Amnion-derived Cellular Cytokine Solution (ACCS), and physiologic cytokine solution (PCS) compositions. The compositions may be used alone or in combination with each other and/or other agents.
1 . A method for treating inhalation injuries in a subject in need thereof comprising administering to the subject an effective amount of a composition selected from the group consisting of extraembryonic cytokine-secreting (ECS) cells, extraembryonic cell-derived cellular cytokine solution, physiologic cytokine solution (PCS), or a combination thereof.
2 . The method of claim 1 wherein the inhalation injury is a thermal burn.
3 . The thermal burn of claim 2 which is caused by heat or steam.
4 . The inhalation injury of claim 1 which is caused by an irritant gas.
5 . The irritant gas of claim 4 which is selected from the group consisting of sulfur dioxide, nitrogen dioxide, ammonia and chlorine.
6 . The inhalation injury of claim 1 which is smoke inhalation.
7 . The extraembryonic cell-derived cellular cytokine solution of claim 1 which is Amnion-derived Cellular Cytokine Solution (ACCS).
8 . The composition of claim 7 wherein the ACCS comprises physiologic concentrations of VEGF, TGFβ2, Angiogenin, PDGF, TIMP-1 and TIMP-2.
9 . The PCS of claim 1 comprising a therapeutic component consisting essentially of physiologic concentrations of VEGF, TGFβ2, Angiogenin, PDGF, TIMP-1 and TIMP-2, and a carrier, wherein the carrier is normal saline, PBS, lactated Ringer's solution or cell culture medium.
10 . The method of claim 1 wherein the ECS cells are Amnion-derived Multipotent Progenitor (AMP) cells.
11 . The compositions of claim 1 which are aerosolized.
12 . The compositions of claim 11 which are administered by nebulizer, vaporizer, or atomizer.