Inhibition of glycerol-3-phosphate acyltransferase (GPAT) and associated enzymes for treatment of viral infections

1. A method of treating a viral infection in a mammal, comprising administering to a mammalian subject in need thereof a therapeutically effective amount of a compound or prodrug thereof, or pharmaceutically acceptable salt or ester of said compound or prodrug wherein the compound is compound of Formula I:

wherein

R 1 and R 2 are each independently —H, —OH, —OR 5 , —OCH 2 C(O)R 5 , —OCH 2 C(O)NHR 5 , —OC(O)R 5 , —OC(O)OR 5 , —OC(O)NHNH—R 5 , —OC(O)NR 5 R 6 , C 1 -C 20 alkyl, cycloalkyl, alkenyl, alkynyl, aryl, arylalkyl, alkylaryl, —CHR 5 OR 6 , —CO(O)R 5 , —C(O)NR 5 R 6 , —CH 2 C(O)R 5 , —CH 2 C(O)NHR 5 , —C(O)R 5 , —CH 2 OR 5 , —C(O)R 5 , —CO(O)R 5 , —C(O)NR 5 R 6 , —CH 2 C(O)R 5 , or —CH 2 C(O)NHR 5 ,

R 5 and R 6 are each independently H, C 1 -C 20 alkyl, cycloalkyl, alkenyl, alkynyl, aryl, arylalkyl, alkylaryl, or optionally contain halogen atoms;

R 3 and R 4 , the same or different from each other, are C 1 -C 20 alkyl, cycloalkyl, alkenyl, alkynyl, aryl, arylalkyl, or alkylaryl; and

wherein the viral infection is by an enveloped virus.

2. The method of claim 1 , which further comprises administering a second compound or prodrug thereof, or pharmaceutically acceptable salt or ester of said compound or prodrug that inhibits a phosphatidic acid synthesis enzyme.

3. The method of claim 1 , further comprising administering to the mammalian subject an inhibitor of acetyl-CoA carboxylase (ACC), an inhibitor of a long chain acyl-CoA synthase, an inhibitor of an elongase, an inhibitor of HMG-CoA reductase, or an inhibitor of mTOR.

4. The method of claim 1 , wherein the compound is

5. The method of claim 1 , wherein the enveloped virus is human cytomegalovirus (HCMV).

6. The method of claim 1 , wherein the enveloped virus is herpes simplex virus-1 (HSV-1).