IP Library Granted Patent US 8,937,215
Granted Patent B2
US 8,937,215 · App. 13/387,760 · Granted Jan 20, 2015

Polynucleotides and polypeptides for increasing desirable plant qualities

Inventors: Sharon Ayal (Kiryat-Ekron, IL); Basia Judith Vinocur (Rechovot, IL); Alex Diber (Rishon-LeZion, IL); Hagai Karchi (Moshav Sitriya, IL); Limor Poraty (Holon, IL)
Assignee: Evogene Ltd.
C12N15/8271C12N15/8247C12N15/8261
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Quick Facts
Patent No.
US 8,937,215
App. No.
13/387,760
Granted
Jan 20, 2015
Kind
B2
Abstract

Polynucleotides and isolated polypeptides, nucleic acid constructs comprising the isolated polynucleotides, transgenic plants expressing same and methods of using same for increasing abiotic stress tolerance, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or nitrogen use efficiency of a plant are disclosed.

Claims (29)

1. A method of increasing tolerance to an abiotic stress condition, yield, biomass, growth rate, vigor, and/or nitrogen use efficiency of a plant as compared to a non-transformed plant which is grown under the same growth conditions, comprising:

(a) over-expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence encoding the polypeptide selected from the group consisting of SEQ ID NOs:548, and 5893-5898, and;

(b) selecting plants resulting from step (a) for an increased tolerance to a salinity stress, an increased growth rate and/or for an increased biomass as compared to a non-transformed plant which is grown under the same growth conditions,

thereby increasing abiotic stress tolerance, yield, biomass, growth rate, vigor, and/or nitrogen use efficiency of a plant as compared to the non-transformed plant which is grown under the same growth conditions.

2. The method of claim 1 , wherein said nucleic acid sequence is selected from the group consisting of SEQ ID NOs: 75, 343, 1221, 1222, 1223, 1224, 1225, 1226, and 1227.

3. The method of claim 1 , further comprising growing the plant expressing said exogenous polynucleotide under the abiotic stress condition.

4. The method of claim 1 , wherein said abiotic stress condition is selected from the group consisting of salinity, drought, water deprivation, flood, low temperature, high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency, nutrient excess, atmospheric pollution and UV irradiation.

5. The method of claim 1 , wherein the yield comprises seed yield or oil yield.

6. A method of growing a crop comprising growing a crop plant transformed with an exogenous polynucleotide which comprises the nucleic acid sequence selected from the group consisting of SEQ ID NOs: 75, 343 and 1221, wherein said crop plant is derived from parent plants that have been transformed to express said exogenous polynucleotide and that have been selected for at least one trait selected from the group consisting of:

an increased tolerance to a salinity stress condition, an increased growth rate, and an increased biomass as compared to a non-transformed plant which is grown under the same growth conditions, and said crop plant which comprises said exogenous polynucleotide having said increased tolerance to said salinity stress, said increased growth rate and/or said increased biomass, thereby growing the crop.

7. A method of growing a crop comprising growing a crop plant transformed with an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide selected from the group consisting of SEQ ID NOs: 548, and 5893-5898, wherein said crop plant is derived from parent plants that have been transformed to express said exogenous polynucleotide and that have been selected for at least one trait selected from the group consisting of: an increased tolerance to a salinity stress condition, an increased growth rate, and an increased biomass as compared to a non-transformed plant which is grown under the same growth conditions, and said crop plant which comprises said exogenous polynucleotide having said increased tolerance to said salinity stress, said increased growth rate and/or said increased biomass, thereby growing the crop.

8. The method of claim 7 , wherein said polypeptide is set forth in SEQ ID NO: 548.

9. The method of claim 7 , wherein said parent plants have been selected for increased biomass as compared to a non-transformed plant which is grown under the same growth conditions.

10. The method of claim 7 , wherein said parent plants have been selected for increased tolerance to a salinity stress condition as compared to a non-transformed plant which is grown under the same growth conditions.

11. The method of claim 7 , wherein said parent plants have been selected for increased growth rate as compared to a non-transformed plant which is grown under the same growth conditions.

12. A method of selecting a plant for increased biomass, growth rate and/or tolerance to salinity stress as compared to a non-transformed plant which is grown under the same growth conditions, the method comprising:

(a) providing plants transformed with an exogenous polynucleotide which comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 75, 343 and 1221,

(b) selecting said plants for increased biomass, growth rate and/or tolerance to a salinity stress condition as compared to a non-transformed plant which is grown under the same growth condition, and

(c) growing a crop of said plant selected in step (b),

thereby selecting the plant for the increased biomass, growth rate and/or tolerance to the salinity stress condition as compared to the non-transformed plant which is grown under the same growth conditions.

13. A method of selecting a plant for increased biomass, growth rate and/or tolerance to salinity stress as compared to a non-transformed plant which is grown under the same growth conditions, the method comprising:

(a) providing plants transformed with an exogenous polynucleotide encoding a polypeptide selected from the group consisting of SEQ ID NOs: 548, and 5893-5898,

(b) selecting said plants for increased biomass, growth rate and/or tolerance to a salinity stress condition as compared to a non-transformed plant which is grown under the same growth condition, and

(c) growing a crop of said plant selected in step (b),

thereby selecting the plant for the increased biomass, growth rate and/or tolerance to the salinity stress condition as compared to the non-transformed plant which is grown under the same growth conditions.

14. The method of claim 13 , wherein said polypeptide is set forth in SEQ ID NO: 548.

15. The method of claim 1 , wherein said polypeptide is set forth in SEQ ID NO:548.

16. The method of claim 7 , wherein said exogenous polynucleotide comprises the nucleic acid sequence selected from the group consisting of SEQ ID NOs: 75, 343, 1221, 1222, 1223, 1224, 1225, 1226, and 1227.

17. The method of claim 13 , wherein said exogenous polynucleotide comprises the nucleic acid sequence selected from the group consisting of SEQ ID NOs: 75, 343, 1221, 1222, 1223, 1224, 1225, 1226, and 1227.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 3, 2012
From: AYAL, SHARON; VINOCUR, BASIA JUDITH; DIBER, ALEX; KARCHI, HAGAI; PORATY-GAVRA, LIMOR
To: EVOGENE LTD.
Reel/Frame 027976/0840 →
Continuity (3)
Provisional Application 61231040 · Aug 4, 2009
Provisional Application 61293743 · Jan 11, 2010
Related Publication 20120131696A1 · May 24, 2012