Antibodies for the detection of integrin complexes in FFPE material
The invention relates to antibodies that are capable to bind the extracellular domain of integrin. Another object of the invention concerns the use of said antibodies for detecting integrins in archival formalin fixed paraffin embedded (FFPE) tissue. The invention also relates to methods for preparing monoclonal rabbit antibodies, wherein the immunogen is an insect expression culture-derived recombinant extracellular integrin domain, and another method for screening anti-integrin antibodies that discriminate between closest integrin homologues and that are especially suited for immunohistochemistry in FFPE material.
1. A monoclonal rabbit antibody, or antigen-binding fragment thereof, against integrin with insect-derived glycosylation pattern and/or integrin with another eukaryotic glycosylation pattern, each comprising at least a light chain variable region (VL) and a heavy chain variable region (VH), wherein the antibody has antigen binding specificity for an exposed epitope of an extracellular integrin domain or extracellular integrin chain domain, and wherein the antibody is able to bind to intact heterodimers of integrin in formalin fixed paraffin embedded (FFPE) material and on viable cells with the substantially same specificity wherein said antibody is selected from the group consisting of:
an antibody that binds to the extracellular domain of integrin αvβ3 and V L comprises the amino acid sequence of SEQ ID NO: 95 (V L -αvβ3) and V H comprises the amino acid sequence of SEQ ID NO: 96 (V H -αvβ3);
an antibody that binds to the extracellular domain of integrin αvβ5 and V L comprises the amino acid sequence of SEQ ID NO: 15 (V L -αvβ5) and V H comprises the amino acid sequence of SEQ ID NO: 16 (V H -αvβ5);
an antibody that binds to the extracellular domain of integrin αvβ6 and V L comprises the amino acid sequence of SEQ ID NO: 135 (V L -αvβ6) and V H comprises the amino acid sequence of SEQ ID NO: 136 (V H -αvβ6);
an antibody that binds to the extracellular domain of integrin αvβ8 and V L comprises the amino acid sequence of SEQ ID NO: 175 (V L -αvβ8) and V H comprises the amino acid sequence of SEQ ID NO: 176 (V H -αvβ8); and
an antibody that binds to the extracellular domain of integrin chain αv and V L comprises the amino acid sequence of SEQ ID NO: 215 (V L -αv) and V H comprises the amino acid sequence of SEQ ID NO: 216 (V H -αv).
2. A polynucleotide which encodes the antibody, or antigen-binding fragment thereof, of claim 1 .
3. A method for obtaining the monoclonal antibody according to claim 1 , comprising
immunizing a rabbit with an immunogen which comprises an extracellular integrin domain with insect-derived glycosylation pattern, optionally coupled as delta-trans membrane form;
obtaining a polyclonal antiserum comprising polyclonal antibodies from said rabbit; and
preparing the monoclonal antibodies.
4. A method for manufacturing a recombinant monoclonal antibody comprising a light chain variable region (V L ) and a heavy chain variable region (V H ) with the steps of:
(a) introducing at least one vector comprising antibody-encoding nucleic acid sequences of
(i) SEQ ID NO: 115 (V L -αvβ3) and SEQ ID NO: 116 (V H -αvβ3),
(ii) SEQ ID NO: 35 (V L -αvβ5) and SEQ ID NO: 36 (V H -αvβ5),
(iii) SEQ ID NO: 155 (V L -αvβ6) and SEQ ID NO: 156 (V H -αvβ6),
(iv) SEQ ID NO: 195 (V L -αvβ8) and SEQ ID NO: 196 (V H -αvβ8), or
(v) SEQ ID NO: 235 (V L -αv) and SEQ ID NO: 236 (V H -αv) into a host cell,
(b) cultivating the host cell in a culture medium, thereby expressing the encoded antibody, and
(c) purifying the expressed antibody.
5. A method for the detection of integrins in formalin fixed paraffin embedded (FFPE) material, comprising contacting said FFPE material with the antibody or an antigen binding fragment thereof of claim 1 .