Method and assay for determining FAS expression
View Patent ↗Methods and immunoassays for the determination of fatty acid synthase (FAS) expression in patients having or suspected of having a proliferative disorder, especially prostate cancer, are disclosed. The sensitive method and assay detect the level of expression of FAS in a biological sample using antibodies that are highly specific for FAS. The method and assay can be used to monitor the progression of cancer, and/or to predict the efficacy of certain treatments or the likelihood of recurrence of the cancer.
1. A method for monitoring the progression of cancer, comprising the steps of:
i. contacting a biological sample from a cancer patient with a first purified anti-FAS antibody that is specific for the peptide encoded by SEQ ID NO 5, under conditions sufficient to form a conjugate of the antibody and FAS protein in the sample;
ii. contacting the conjugate formed in step (a) with a second anti-FAS antibody, wherein the second antibody is tagged with a detectable label;
iii. determining the amount of FAS protein present in the sample; and
iv. correlating the amount of FAS present in the sample with aggressiveness of the cancer;
wherein the presence of a high level of FAS in the sample is indicative of an aggressive form of the cancer.
2. The method of claim 1 , wherein the biological sample is serum.
3. The method of claim 1 , wherein the detectable labels are fluorescent labels.
4. The method of claim 1 , wherein the first antibody is disposed on a substrate.
5. The method of claim 4 , wherein the substrate is a microtiter plate.