Blue transgenic fluorescent ornamental fish
View Patent ↗The present invention relates to transgenic blue ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
1. A transgenic fluorescent zebrafish that exhibits a fluorescence and comprises chromosomally integrated expression cassette encoding a blue fluorescent protein, wherein the zebrafish comprises the “Blue zebrafish 1 transformation event,” sperm comprising the Blue zebrafish 1 transformation event having been deposited as ECACC accession no. 11012802.
2. The transgenic zebrafish of claim 1 , further defined as a fertile, transgenic zebrafish.
3. The transgenic zebrafish of claim 1 , further defined as a transgenic Golden zebrafish.
4. The transgenic zebrafish of claim 1 , wherein the fish is homozygous for the integrated expression cassette.
5. The transgenic zebrafish of claim 1 , wherein the fish is heterozygous for the integrated expression cassette.
6. A method of providing a transgenic fluorescent zebrafish that exhibits a fluorescence to the ornamental fish market, comprising obtaining a transgenic zebrafish in accordance with claim 1 , and distributing the fish to the ornamental fish market.
7. The method of claim 6 , wherein the fish are distributed by a grower to a commercial distributor.
8. The method of claim 6 , wherein the fish are distributed by a grower or a commercial distributor to a retailer.
9. The method of claim 8 , wherein the retailer is a multi-product retailer having an ornamental fish department.
10. A method of producing a transgenic fluorescent zebrafish that exhibits a fluorescence, the method comprising:
(a) obtaining a zebrafish that exhibits a fluorescence and comprises chromosomally integrated expression cassette encoding a blue fluorescent protein, wherein the zebrafish comprises the “Blue zebrafish 1 transformation event,” sperm comprising the Blue zebrafish 1 transformation event having been deposited as ECACC accession no. 11012802; and
(b) breeding the obtained zebrafish with a second zebrafish to provide a transgenic zebrafish comprising the Blue zebrafish 1 transformation event.
11. The method of claim 10 , wherein the second zebrafish is a non-transgenic zebrafish.