IP Library Granted Patent US 8,580,970
Granted Patent B2
US 8,580,970 · App. 13/412,270 · Granted Nov 12, 2013

Copper-catalysed ligation of azides and acetylenes

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Quick Facts
Patent No.
US 8,580,970
App. No.
13/412,270
Granted
Nov 12, 2013
Kind
B2
Abstract

A copper catalyzed click chemistry ligation process is employed to bind azides and terminal acetylenes to provide 1,4-disubstituted 1,2,3-triazole triazoles. The process comprises contacting an organic azide and a terminal alkyne with a source of reactive Cu(I) ion for a time sufficient to form by cycloaddition a 1,4-disubstituted 1,2,3-triazole. The source of reactive Cu(I) ion can be, for example, a Cu(I) salt or copper metal. The process is preferably carried out in a solvent, such as an aqueous alcohol. Optionally, the process can be performed in a solvent that comprises a ligand for Cu(I) and an amine.

Claims (31)

1. A copper catalyzed process for preparing a 1,4-disubstituted 1,2,3-triazole comprising:

contacting a solution of an organic azide and a terminal alkyne with a source of catalytic Cu(I) ion for a time sufficient to form by cycloaddition a 1,4-disubstituted 1,2,3-triazole.

2. The process of claim 1 wherein the solution further comprises at least one ligand comprising at least one functional group selected from the group consisting of a nitrile, an isonitrile, a primary amine, a secondary amine, a tertiary amine, a nitrogen bearing heterocycle, a carboxylate, a halide, an alcohol, a thiol, a sulfide, a phosphine, and a phosphite.

3. The process of claim 1 wherein the solution is an aqueous solution.

4. A copper catalyzed process for preparing a 1,4-disubstituted 1,2,3-triazole comprising:

contacting a solution of an organic azide and a terminal alkyne with a source of catalytic Cu(I) ion for a time sufficient to form by cycloaddition a 1,4-disubstituted 1,2,3-triazole;

wherein the source of Cu(I) ion comprises a Cu(I) salt.

5. The process of claim 4 wherein the solution further comprises at least one ligand comprising a functional group selected from the group consisting of a nitrile, an isonitrile, a primary amine, a secondary amine, a tertiary amine, a nitrogen bearing heterocycle, a carboxylate, a halide, an alcohol, a thiol, a sulfide, a phosphine, and a phosphite.

6. The process of claim 4 wherein the solution further comprises at least one ligand comprising a nitrogen bearing heterocycle group.

7. The process of claim 4 wherein the solution further comprises at least one polyvalent ligand comprising one or more functional groups selected from the group consisting of a nitrile, an isonitrile, a primary amine, a secondary amine, a tertiary amine, a nitrogen bearing heterocycle, a carboxylate, a halide, an alcohol, a thiol, a sulfide, a phosphine, and a phosphite.

8. The process of claim 4 wherein the solution further comprises at least one polyvalent ligand comprising one or more nitrogen bearing heterocycle groups.

9. The process of claim 4 wherein the solution further comprises at least one polyvalent ligand comprising one or more alcohol groups.

10. The process of claim 4 wherein the Cu(I) salt is selected from the group consisting of CuI, CuOSO 2 CH 3 , and [Cu(CH 3 CN) 4 ]PF 6 .

11. The process of claim 4 wherein the solution further comprises an amine.

12. The process of claim 4 wherein the solution is an aqueous solution.

13. The process of claim 12 wherein the aqueous solution further comprises an alcohol.

14. The process of claim 12 wherein the aqueous solution is free from an organic co-solvent.

15. A copper catalyzed process for preparing a 1,4-disubstituted 1,2,3-triazole comprising:

contacting a solution of an organic azide and a terminal alkyne with a source of catalytic Cu(I) ion for a time sufficient to form by cycloaddition a 1,4-disubstituted 1,2,3-triazole;

wherein the source of catalytic Cu(I) is Cu(II) ion in combination with a reducing agent.

16. The process of claim 15 wherein the reducing agent comprises sodium ascorbate.

17. The process of claim 15 wherein the reducing agent comprises glutathione.

18. The process of claim 15 wherein the reducing agent comprises cysteine.

19. The process of claim 15 wherein the Cu(II) ion is provided as a Cu(II) salt.

20. The process of claim 15 wherein the solution is an aqueous solution.

21. The process of claim 20 wherein the aqueous solution is free from an organic co-solvent.

22. The process of claim 15 wherein the solution comprises a primary amine buffer.

23. The process of claim 15 wherein the solution further comprises a ligand for Cu(I) ion.

24. The process of claim 23 wherein the ligand for Cu(I) ion comprises one or more functional groups selected from the group consisting of a nitrile, an isonitrile, a primary amine, a secondary amine, a tertiary amine, a nitrogen bearing heterocycle, a carboxylate, a halide, an alcohol, a thiol, a sulfide, a phosphine, and a phosphite.

25. The process of claim 15 wherein the solution further comprises a ligand for Cu(I) comprising one or more nitrogen bearing heterocycle groups.

26. The process of claim 15 wherein the solution further comprises at least one polyvalent ligand comprising one or more alcohol groups.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 14, 2019
From: CEMPRA PHARMACEUTICALS, INC.
To: TETARD, INC.
Reel/Frame 050056/0731 →
CONFIRMATORY LICENSE Recorded Apr 11, 2014
From: THE SCRIPPS RESEARCH INSTITUTE
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 032670/0615 →