Instruments and methods for mixing the contents of a detection chamber
View Patent ↗A receptacle having interconnected chambers arranged to permit multiple process steps to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.
1. A method of processing a sample in a receptacle having a plurality of interconnected chambers, including a detection chamber, the method comprising the steps of:
(a) moving a compression element positioned between the receptacle and a detector into engagement with a flexible portion of the detection chamber, thereby depressing the flexible portion to agitate the contents of the detection chamber or to move the contents of the detection chamber into a directly connected chamber of the receptacle, wherein an actuator moves the compression element relative to the detector;
(b) transmitting excitation light from a light source of the detector through the compression element and to the detection chamber; and
(c) detecting a light signal transmitted through the compression element from the contents of the detection chamber with a signal detecting element of the detector.
2. The method of claim 1 , wherein the actuator is a pneumatic actuator.
3. The method of claim 1 , wherein the light signal is detected through an optically transmissive window of the compression element during step (c).
4. The method of claim 1 , wherein the detector remains stationary during the method.
5. The method of claim 4 , wherein the compression element has a tubular body in sliding engagement with a light-directing conduit of the detector.
6. The method of claim 1 , wherein the contents of the detection chamber are moved into the directly connected chamber during step (a).
7. The method of claim 1 , wherein one or more fluorescent signals emitted by the contents of the detection chamber are detected during step (c).
8. The method of claim 1 , further comprising the step of applying thermal energy to the detection chamber on a side of the receptacle opposite the side engaged by the compression element.