Glutathione S-transferase omega 1 wild type specific antibody
View Patent ↗The invention relates to a novel antibody which binds to wild type (wt) Glutathione S-transferase Omega 1 (wtGSTO1) but not to mutant (mut) GSTO1 and methods and uses based on the antibody. The antibody is based on novel haptens and immunogens.
1. A polypeptide hapten having a structure selected from the group consisting of
(SEQ ID NO: 2)
Cys-Lys-Glu-Asp-Tyr-Ala-Gly-Leu-Lys; and
(SEQ ID NO 6)
Lys-Glu-Asp-Tyr-Ala-Gly-Leu-Lys-Cys;
wherein a crosslinking group is attached to the sulphur atom of Cys.
2. An immunogen comprising the polypeptide hapten of claim 1 coupled to an immunogenicity conferring carrier molecule.
3. An isolated antibody raisable from the immunogen of claim 2 which specifically binds to wtGSTO1 (SEQ ID NO: 3)and does not bind to mutGSTO1 (SEQ ID NO: 4).
4. The isolated antibody of claim 3 which is a monoclonal antibody.
5. A method for the detection and/or determination of wtGSTO1 in an in vitro patient sample, the method comprising:
contacting the patient sample with an antibody of claim 4 and means to detect binding by said antibody with a detecting agent,
detecting and/or determining any signal produced by the detecting agent means by reference to one or more calibrators,
in which the presence of a detectable or quantifiable signal indicates the presence or amount of wtGSTO1.
6. A kit for detecting and/or determining the presence of wtGSTO1 (SEQ ID NO: 3), in a sample, the kit comprising the antibody of claim 3 and means to detect binding by said antibody.
7. The kit of claim 6 , wherein the antibody is a monoclonal antibody.
8. The kit of claim 6 , further comprising a detecting agent that recognizes a common epitope of wtGSTO1 (SEQ ID NO: 3) and mutGSTO 1 (SEQ ID NO: 4).
9. The kit of claim 7 , comprising a detecting agent that recognizes a common epitope of wtGSTO1 (SEQ ID NO: 3) and mutGSTO1 (SEQ ID NO: 4).
10. The kit of claim 8 , further comprising an antibody which binds both wtGSTO1 (SEQ ID NO: 3) and mutGSTO1 (SEQ ID NO: 4); and the kit additionally comprises one or more detecting agents.
11. The kit of claim 7 , further comprising an antibody which binds both wtGSTO1 (SEQ ID NO: 3) and mutGSTO1 (SEQ ID NO: 4); and the kit additionally comprises one or more detecting agents.
12. The antibody of claim 3 wherein the isolated antibody has a cross-reactivity of less than 0.1% for mutGSTOl (SEQ ID NO: 4), when compared with wtGSTO1 (SEQ ID NO: 3).
13. The antibody of claim 4 wherein the isolated antibody has a cross-reactivity of less than 0.1% for mutGSTO1 (SEQ ID NO: 4), when compared with wtGSTO1 (SEQ ID NO: 3).
14. The kit of claim 6 wherein the isolated antibody has a cross-reactivity of less than 0.1% for mutGSTO1 (SEQ ID NO: 4), when compared with wtGSTO1 (SEQ ID NO: 3).
15. The kit of claim 7 wherein the isolated antibody has a cross-reactivity of less than 0.1% for mutGSTO1 (SEQ ID NO: 4), when compared with wtGSTO1 (SEQ ID NO: 3).