Method for the reduction of cross talk in multiplex capillary electrophoresis
View Patent ↗The invention is a high-throughput multi-capillary system utilizing a 2-dimensional mask onto which the detection windows of the capillaries are lined up in alternating columns and rows. The image of the capillaries is projected onto a 2-dimensional detection system, such as a charge-coupled-detector (CCD) array. Each row and column of the capillary detection windows is imaged in a separate area of the CCD array, which results in reduced cross-talk between capillaries.
1. A multi-capillary electrophoresis detection apparatus having side-by-side capillary detection windows disposed in a plane, a light source positioned to direct a beam of light through each capillary detection window to induce a fluorescent emission from any samples in the capillaries, a light collection lens to direct fluorescent emissions from said samples to a two-dimensional array detector positioned to receive said emissions from said collector lens, the improvement comprising:
arranging said side-by-side capillary detection windows in a two-dimensional mask so that the detection window of any given capillary (n) is positioned in a different row and column than that of any immediately adjacent capillary detection window (n+1, n−1);
and each row contains more than one capillary detection window.
2. The apparatus of claim 1 in which said mask comprises at least two rows and at least four columns; said detection window of each capillary n is in a different row and column from any immediately adjacent capillary n+1 or n−1; and each row contains at least two capillary detection windows.
3. The apparatus of claim 1 in which said mask comprises at least three rows and nine columns said detection window of any capillary n is in a different row and column from any immediately adjacent capillary detection window n+1 or n−1; and each row contains at least three capillary detection windows.
4. The apparatus of claim 1 in which said mask comprises at least four rows and at least sixteen columns; said detection window of each capillary n is in a different row and column from any adjacent capillary n+1 and n−1; and each row contains at least 4 capillary detection windows.
5. The apparatus of claim 1 , in which the image of fluorescent light from capillary detection windows of multiple capillaries are projected onto at least two rows and four columns of said two-dimensional array detector and each row contains the projection of at least two capillary detection windows.